A Lectin Receptor Kinase Positively Regulates ABA Response During Seed Germination and Is Involved in Salt and Osmotic Stress Response

다국어 초록 (Multilingual Abstract)

Lectin receptor-like kinases (LecRK) are widespread

in higher plants; however, little is known about their

physiological roles. In this study, At1g70130 (designated

LecRK-b2), an Arabidopsis LecRK gene, has been investigated.

LecRK-b2 was predominantly expressed during seed

germination, and its expression was ceased following

germination. The expression of LecRK-b2 was induced by

abscisic acid (ABA), salt, and osmotic stress. LecRK-b2 lossof-

function mutation slightly reduced the ABA sensitivity

during seed germination, and this reduced sensitivity was

demonstrated not due to lower ABA accumulation level in

the seeds. Dual-luciferase transient expression assay confirmed

that the transcription factor ABSCISIC ACID

INSENSITIVE3 (ABI3) could activate the luciferase under

driving of LecRK-b2 promoter. LecRK-b2 transcription level

was found to be down-regulated in abi3 during seed

germination. Furthermore, LecRK-b2 loss-of-function mutation

reduced the salt and osmotic sensitivity during early

development stage of Arabidopsis. Taken together, these

results suggest that LecRK-b2 functions as a positive

regulator of the ABA response during the seed germination

and is involved in salt and osmotic stress response in the

early development stage.

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