배양된 인간 골막기원세포의 조골세포 분화과정에서 골기질 형성정도와 혈관내피세포성장인자 신호와의 상관관계|RISS 상세보기

다국어 초록 (Multilingual Abstract)

Angiogenesis is a essential part for bone formation and bone fracture healing. Vascular endothelial growth factor (VEGF), one of the most important molecules among many angiogenic factors, is a specific mitogen for vascular endothelial cells. VEGF-mediated angiogenesis is required for bone formation and repair. However, the effect of VEGF on osteoblastic cells during osteogenesis is still controversial. In recent days, substantial progress have been made toward developing tissue-engineered alternatives to autologous bone grafting for maxillofacial bony defects. Periosteum has received considerable interest as a better source of adult stem cells. Periosteum has the advantage of easy harvest and contains various cell types and progenitor cells that are able to differentiate into a several mesenchymal lineages, including bone. Several studies have reported the bone formation potential of periosteal cells, however, the correlation between VEGF signaling and cultured human periosteal cell-derived osteogenesis has not been fully investigated yet. The purpose of this study was to examine the correlation between VEGF signaling and cultured human periosteal-derived cells osteogenesis. Periosteal tissues of $5\;{\times}\;20\;mm$ were obtained from mandible during surgical extraction of lower impacted third molar from 3 patients. Periosteal-derived cells were introduced into the cell culture and were subcultured once they reached confluence. After passage 3, the periosteal-derived cells were further cultured for 42 days in an osteogenic inductive culture medium containing dexamethasone, ascorbic acid, and ${\beta}-glycerophosphate$. We evaluated the alkaline phosphatase (ALP) activity, the expression of Runx2 and VEGF, alizarin red S staining, and the quantification of osteocalcin and VEGF secretion in the periosteal-derived cells. The ALP activity increased rapidly up to day 14, followed by decrease in activity to day 35. Runx2 was expressed strongly at day 7, followed by decreased expression at day 14, and its expression was not observed thereafter. Both VEGF 165 and VEGF 121 were expressed strongly at day 35 and 42 of culture, particularly during the later stages of differentiation. Alizarin red S-positive nodules were first observed on day 14 and then increased in number during the entire culture period. Osteocalcin and VEGF were first detected in the culture medium on day 14, and their levels increased thereafter in a time-dependent manner. These results suggest that VEGF secretion from cultured human periosteal-derived cells increases along with mineralization process of the extracellular matrix. The level of VEGF secretion from periosteal-derived cells might depend on the extent of osteoblastic differentiation.

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참고문헌 (Reference)

1 "Vesiculo-vacuolarorganelles and the regulation of venule permeability tomacromolecules by vascular permeability factor, histamine,and serotonin" 183 : 1981-, 1996

2 "Vascularendothelial growth factor (VEGF) and its receptors" 13 : 9-, 1999

3 "Vascular permeability factor/vascularendothelial growth factor : a critical cytokine in tumorangiogenesis and a potential target for diagnosis and ther-apy" 20 : 4368-, 2002

4 Street J, "Vascular endothelialgrowth factor stimulates bone repair by promoting angio-genesis and bone turnover" 99 : 9656-, 2002

5 "VEGF couples hyper-trophic cartilage remodeling, ossification and angiogenesisduring endochondral bone formation" 5 : 623-, 1999

6 "The osteogenicpotential of adipose-derived stem cells for the repair ofrabbit calvarial defects" 56 : 543-, 2006

7 "Prevention offracture healing in rats by an inhibitor of angiogenesis" 29 : 560-, 2001

8 "Periosteal cells in bone tis-sue engineering" 9 : S45-, 2003

9 "Osteogenic potential ofcultured periosteal cells in a distracted bone gap in rab-bits" 78 : 68-, 1998

10 "Osteogenic dif-ferentiation of human bone marrow-derived mesenchymalstem cells cultured on alumina ceramics" 28 : 72-, 2004