XS-E가 아토피피부염이 유발된 NC/Nga Mice의 피부상태에 미치는 영향

다국어 초록 (Multilingual Abstract)

This study reports significant improvement of atopic dermatitis condition as a result of experiment using Xanthium strumarium L. extract (XS-E) at the dorsal skin of induced atopic dermatitis Nc/Nga mice. Skin clinical score has decreased (2.75±0.85, *p<0.05), showing visible change of skin condition. IgE (***p<0.001) and IgG1 (2522.00±32.80, ***p<0.001) in plasma also decreased significantly. mRNA (gene expression) level increased (RQ=2.75±0.10, ***p<0.001) within skin tissue of CD4+CD25+Foxp3+ Treg cell that’s activated by XS-E dosage, thereby discovering that there is an effect of suppressing proliferation and viability of Th2 cell, eosinophils, mast cell and inflammatory cell. Upon examining cells permeated with H&E and toluidine blue staining technique, thickness of epidermis and mast cell’s permeation decreased, and the result of examining the distribution of CCR 3+ eosinophils within ALN showed that it’s level fell down to that of wild type (normal group, NC/Nga-WT). By such results, it is suggested that XS-E is highly effective on atopic dermatitis, and it is considered that continued quantitative research and case study of clinical research such as effect of cell number in individual tissues or change of total cell number are necessary.

목차 (Table of Contents)

• Abstract
• 1. INTRODUCTION
• 2. MATERIALS AND METHOD
• 3. RESULTS AND DISCUSSION
• 4. CONCLUSION
• REFERENCES

참고문헌 (Reference)

1 이영철, "천식 모델 생쥐에서 半夏의 CD4+CD25+ 조절 T 세포 상승 및 CD3+CCR3+Th2 세포 침윤 억제 효과" 대한본초학회 24 (24): 73-78, 2009

2 Shih, I. L., "The production of poly- (gammaglutamic acid) from microorganisms and its various applications" 79 : 207-225, 2001

3 Lee, B. H., "The Effect of Changiga on Immune Cell & Serum OA-specific IgE in BALF in Rat Asthma Model" 5 : 93-105, 2001

4 Park, E. J., "Suppression of Spontaneous Dermatitis in NC/Nga Murine Model by PG102 Isolated from Actinidia arguta" 127 : 1154-1160, 2007

5 서영배, "Study on the Effects of Xanthii Fructus about the anti-allergic Action on IC-2 Cells." 대한본초학회 17 (17): 4-54, 2002

6 Rousset, F., "Shifts in interleukin-4and interferon-gamma production by T cells of patients with elevated serum IgE levels and the modulatory effects of these lymphokines on spontaneous IgE synthesis" 87 : 58-69, 1991

7 Hashimoto, Y., "Scratching of their skin by NC/Nga mice leads to development of dermatitis" 76 : 783-794, 2004

8 Bayry, J., "Rescuing CD4+CD25+ regulatory T-cell functions in rheumatoid arthritis by cytokine-targeted monoclonal antibody therapy" 12 : 548-552, 2007

9 Wan, Y. Y, "Regulatory T cells: immune suppression and beyond" 7 : 204-210, 2010

10 Montagne, A., "Quantification of ovine cytokine gene expression by a competitive RT-PCR method" 253 : 83-93, 2001

11 Ohmen, J. D., "Overexpression of IL-10 in atopic dermatitis. Contrasting cytokine patterns with delayed-type hypersensitivity reactions" 154 : 1956-1963, 1995

12 Miyara, M., "Natural regulatory T cells:Mechanisms of suppression" 13 : 108-116, 2007

13 Kleij, H. P., "Murine model for non-IgE-mediated Asthma" 28 : 115-125, 2004

14 Kim, H. S, "Isolation and Antimicrobial of Xanthium strumarium L" 25 : 183-188, 1997

15 Oh, M. J, "Ingibitory Effect of Mast Cell-Mediated Immediate-Type Allergic Reaction by Xanthii Fructus" Wonkwang University 2002

16 Kim, Y. S., "Improvement of Atopic Dermatitis in NC/Nga Mice by Topical Application of CpG Phosphodiester-ODN" 144 : 315-324, 2007

17 Dogru, M., "Impression cytology in atopic dermatitis" 105 : 1478-1484, 1998

18 Matsumoto, M., "IgE hyperproduction through enhanced tyrosine phosphorylation of Janus kinase 3 in NC/Nga mice, a model for human atopic dermatitis" 162 : 1056-1063, 1999

19 LEE, M. Y, "Hydroquinone, a metabolite of benzene, enhances interleukin-4 production in CD4+ T cells and increases IgE levels in antigen-primed mice" Chunnam National University 2002

20 Sicherer, S. H., "Food hypersensitivity and atopic dermatitis: Pathophysiology, epidemiology, diagnosis, and management" 104 : 114-122, 1999

21 Jun, H., "Effect of Tacrolimus Hydrate (FK506) Ointment on Spontaneous Dermatitis in NC/Nga Mice" 76 : 175-183, 1998

22 Jöhrens, K., "Different T-bet expression patteerns characterize particular reactive lymphoid tissue lesions" 48 : 343-352, 2006

23 Matsuda, H., "Development of atopic dermatitis like skin lesion with IgE hyperproduction in NC/Nga mice" 9 : 461-466, 1997

24 Hori, S., "Control of regulatory T cell development by the transcription factor Foxp3" 14 : 1030-1031, 2003

25 Danniel, W. W, "Biostatistics: a foundation for analysis in the health science" WILEY EDITION Publisher 136-146, 2005

26 Coffman, R. L., "B cell stimulatory factor enhances the IgE response of lipopolysaccharide-activate B cell" 136 : 4538-4541, 1986

27 Galli, S. J, "Allergy" 10 : 93-95, 2006

28 De Groot, A. S., "Activation of natural regulatory T cells by IgG Fc-derived peptide “Tregitopes”" 8 : 112-120, 2008

29 Miyamoto, K., "A synthetic glycolipid prevents autoimmune encephalomyelitis by inducing TH2 bias of natural killer T cells" 413 : 531-534, 2001

30 Yamamoto, M., "A novel atopic dermatitis model induced by topical application with dermatophagoides farinae extract in NC/Nga mice" 56 : 139-148, 2007

31 Kim, S. H., "90 days Oral Repeated Dose Toxicity and Genotoxicity study of a Xanthium strumarium L"