황산마그네슘이 인간제대정맥내피세포의 VEGF와 Caspase-3에 미치는 영향 = Original Reports : Effects of magnesium sulfate on VEGF and Caspase-3 of HUVECs (Human umbilical vein endothelial cells)

다국어 초록 (Multilingual Abstract)

Objectives : To determine the effects of magnesium sulfate on Caspase-3 and Vascular endothelial growth factor (VEGF) of human umbilical vein endothelial cells (HUVECs) under presence or absence of Tumor necrosis factor (TNF-α). Methods : HUVECs were isolated from normal term umbilical cords and cultured in several physiolo-gically relevant concentrations of magnesium sulfate with or without exposure of TNF-α. The concentrations of VEGF and caspase-3 were estimated by colorimetric assay and ELISA assay, respectively. Results : The concentration of VEGF in HUVECs significantly increased in the presence of TNF-α compared with in the absence of TNF-α. However, the concentration of VEGF did not show significant difference in several concentrations of magnesium sulfate concentrations with addition of TNF-α and it showed the lowest concentration under 4 mM and 8 mM of magnesium sulfate concentration without addition of TNF-α. The concentration of caspase-3 in HUVECs did not show statistically significant difference with the addition of TNF-α and magnesium sulfate. Conclusion: TNF-α induce HUVECs to stimulate the VEGF, and magnesium sulfate might not have the effects on the expression of VEGF with addition of TNF-α. However, the concentration for treatment of magnesium sulfate inhibits the expression of VEGF without addition of TNF-α. Magnesium sulfate might not have an effect of the expression of caspase-3 with or without addition of TNF-α.