A kinetic model, considering the inhibition effects of both the substrate and the product was developed for the hydrolysis of U.K.P. by cellulose from Trichoderma reesei QM 9414.
When the initial concentrations became over 25 G/L and 7.8 G/L for cell...
A kinetic model, considering the inhibition effects of both the substrate and the product was developed for the hydrolysis of U.K.P. by cellulose from Trichoderma reesei QM 9414.
When the initial concentrations became over 25 G/L and 7.8 G/L for cellulose and cellobiose respectively, the substrate inhibition was observed to be significant.
In this paper, the kinetic constants such as Michaelis-Menten constant, the maximum reaction velocity and the inhibition constant, were presented for each reaction; cellulose to cellobiose and cellobiose to glucose. Also the theoretically computed concentrations of the substrate and product with the kinetic constants were in good agreement with the obtained experimental ones until the 24 hours of reaction time.