Nucleus accumbens septi내의 ³H-Spiperone분포에 대한 전자현미경적 연구 = Electron Microscopic Autoradiographic Study on the Distribution of ³H-Spiperone in the Nucleus accumbens septi

국문 초록 (Abstract)

뇌 중격측좌핵내의 dopamine 수용체의 미세구조를 밝히려고 실험하였다. 흰쥐를 뇌정위고정대에 고정하고 측뇌실(좌표 앞+6.0㎜, 옆+1.2㎜, 높이-3㎜, 측각 15° ; De Groot 도보)에 50μCi의 방사능을 지닌 1㎍의 ³H-spiperone을 서서히 주입한 후 2시간이 지나서 뇌를 고정하고 전자현미경적 자기방사술을 시행하였다. 감광제는 Ilford L₄를 사용했고 microdol-X로 현상 관찰하여 다음과 같은 결론을 얻었다.
1. 대부분의 표지는 세표체, 수상돌기, 극돌기에 나타났는데 이들은 한 세포종류에 속하며 dopamine신경종말에 대한 연접 후 구조라고 생각된다.
2. 드물게는 신경종말에 표지되는데 이는 dopamine 종말로 생각된다.
3. 세포소기관 별로는 내형질세망에 표지가 많았다.

다국어 초록 (Multilingual Abstract)

Various morphological studies on he nucleus accumbens septi have shown the peculiar position between the limbic and striatal components. In this study, dopamine receptors in the nucleus accumbens septi was autoradiographically studied with ³H-spiperone.
Rats were fixed on the stereotaxic apparatus(David Kopf Inc.), and 0.2ml/1㎍/50μCi of ³H-spiperone was perfused into the lateral ventricle of each rat. Perfusion velocity was controlled very slowly during 80minutes. Two hours following the perfusion, rats were transcardially perfused with 500ml of 1% glutaraldehyde-1%
paraformaldehyde. After the fixation time of 2 hours, brains are removed and refixed within the same perfusion fixativesovernight. In the nextday, nucleus accumbens septi was punched out, immersed in the 2% osmium tetroxide, dehydrated and embeded in the araldite mixture. Ultrathin section were stained with uranyl acetate-lead citrated on the collodionated slide glass, coated with carbon particles, dipped in the diluted Ilford L4 photographic emulsion. Following the exposure time of 2 to 4 months, samples were developed with microdol-X. Retrieved sections were put on the grids and observed with JEM100 CX electron microscope. Results observed were as follows :
1. Lables were found mostly on the soma, dendrite or dendritic spines. They might be postsynaptic structures to the dopaminergic terminals.
2. Within the neuronal structures, labels are located over endoplasmic reticula, microtubules, membranes, mitochondria, etc.
3. Some lables were found over the axon terminals. These terminals were interpreted as dopaminergic ones.
4. Possibility of correlation of lables over endoplasmic reticula with the formation or movement of receptor site was discussed.