Alkalophilic Bacillus sp. KCTC 1911의 GI 生成조건 = Studies on the glucose isomerase produced by alkalophilic Bacillus sp. KCTC 1911

다국어 초록 (Multilingual Abstract)

Glucose isomerase (E.C., 5.3.1.5) which reversibly catalyzes reaction between D-glucose and D-fructose was demonstrated in cellfree extracts of alkalophilic Bacillus sp. KCTC 1911 isolated from soil.
Numerous studies on glucose isomerase by acidophiles or neutrophiles have been reported by several workers. However, the reports on glucose isomerase by alkalophilic organisms were not found to date. The author screened and isolated a alkalophilic bacterium from soil which produced a quantitative amount of the glucose isomerased in the alkaline medium.
The characteristic point of this bacterium was especially good growth in alkaline media, but nogrowth was observed in neutral media such as nutrient broth and identified as Bacillus sp. KCTC 1911 by a series of studies on its physiological, cultural characteristics.
The optimum pH and temperature for the growth of the organism were in the ranges 10.4-10.5 and 30℃ respectively.
D-xylose is found to be effective carbon source for the formation of the glucose isomerase. On the countrary, melizitose, galactose, lactate, melibiose, gluconate, etc., were ineffective or less effective on the enzyme formation.
Yeast extract could be used as good nitrogen sources and corn steep liquor was found to be most effective for the formation of the enzyme.
The addition of Co^(++) to medium remarkably improved the activity of the cell to convert glucose into fructose.
The enzyme was activated by 0.08M Mg^(++) but only activated by 1.4mM Co^(++).
The temperature optimum for the enzyme activity was about 65℃ and the pH optimum was between 7.2-7.4.

목차 (Table of Contents)

• Ⅰ. 서론
• Ⅱ. 재료 및 방법
• １. 균의 분리 및 선정
• ２. 배양방법
• ３. 균주의 특성과 동정
• ４. 효소활성 측정방법
• ５. GI 생성과정
• Ⅲ. 결과 및 고찰
• １. 균주의 특성과 동정
• ２. GI 생성조건
• Ⅳ. 결론
• 참고문헌
• 영문초록