Tween-80 Can Enlarge the Swarming Haloes of Human-Pathogenic Bacteria

다국어 초록 (Multilingual Abstract)

Bacterial swarming is considered as a good in vitro model for colonization on and adhesion to the epithelial surfaces, and some efforts to enlarge swarming haloes have been made. Recently, Tween-80, a commercially available anionic surfactant, was reported to be able to enlarge the swarming haloes of some non-human-pathogenic bacteria on the surfaces of agars with relatively high viscosity by reducing surface tension, In this study, we attempted to observe whether Tween-80 can also enlarge the swarming haloes of some human-pathogenic bacteria. Proteus mirabilis, Serratia marcescens and Vibrio parahaemolyticus could swarm on the surfaces of semisolid agars containing over 0.7% Bacto agar, and their swarming haloes were 2.7-, 1.6- and 9.0-fold enlarged by Tween-80. In contrast, Escherichia coli, Vibrio vulnificus and Vibrio cholerae could swarm only on the surfaces of semisolid agars containing 0.3% Bacto agar, and their swarming haloes were 1.5-, 1.5- and 1.4-fold enlarged by Tween-80. Accordingly, Tween-80 can be particularly useful in enlarging the swarming haloes of V. parahaemolyticus on the surfaces of semisolid agars with relatively high viscosity.