하수오 물 추출물이 마우스 골다공증에 미치는 영향 = Antiosteoporotic Effects of Polygoni Multiflori Radix (PMR) in Ovariectomized (OVX)-Induced Osteoporosis ddY Mice

다국어 초록 (Multilingual Abstract)

Polygoni Multiflori Radix (PMR), a dried root tuber of Polygonum multiflorum Thunberg with bioactivities in bone metabolism is one of the most famous tonic traditional medicines. To observe in vivo anti-osteoporotic efficacy of PMR extracts, we orally administered once a day for 28 days (Qd × 28) to bilateral ovariectomized (OVX)-induced osteoporosis ddY mice after 1 week of recovery periods at 125, 250 and 500 mg/kg (of body weight). A positive control drug, Alendronate (FOSA) 10 mg/kg-dosing group was added. As results of OVX-induced osteoporotic process, estrogen-deficient osteoporotic changes were also dramatically decreased in all PMR extracts-dosing groups. Especially middle dosage of PMR extracts, 250 mg/kg constantly and significantly (p < 0.01 or p < 0.05) inhibited the loss of bone strength and bone quality. Based on the results, it was concluded that PMR extracts (125, 250 or 500 mg/kg;orally dosing) has relatively good favorable effect to prevention and/or treatment of OVX-induced osteoporosis. Therefore, although the efficacy was slighter than that of Alendronate on the inhibition of bone loss, it is expected that PMR extracts will be promising as a new anti-osteoporotic agents for prevent the fracture induced in osteoporotic patients because natural herbal medicine origin PMR extracts will be dose not show serious side effects especially the problem in upper alimentary irritation by bisphosphonate and hypercalcaemia of parathyroid hormone analogs.

참고문헌 (Reference)

1 류종훈, "한방약리학" 신일상사 2006

2 이해남, "한국 여성의 요골 골밀도치에 의한 골다공증의 빈도 및 폐경에 따른 변화" 대한산부인과학회 45 (45): 12-12, 2002

3 김민정, "하수오와 백수오가 난소적출로 유발된 흰쥐의 골다공증 예방효과에 미치는 영향" 대한본초학회 19 (19): 23-34, 2004

4 조수현, "폐경과 골다공증" 35 : 587-598, 1992

5 대한정형외과학회, "정형외과학" 최신의학사 13-14, 1992

6 강소신의학원, "신편중약대사전" 의성당 862-865, 1992

7 이동선, "두충[Eucommia ulmoides Oliver.] 조성물이 골다공증에 미치는 효과" 16 : 614-619, 2001

8 지형준, "대한약전 및 대한약전외 한약규격주해" 한국메디칼 인덱스사 266-267, 1998

9 Bilston LE, "Zoledronic acid improves the mechanical properties of normal and healing bone" 17 : 716-718, 2002

10 Ederveen AG, "Tibolone, a steroid with a tissuespecific hormonal profile, completely prevents ovariectomyinduced bone loss in sexually mature rats" 14 : 1963-1970, 1999

11 Rodan GA, "Therapeutic approaches to bone diseases" 289 : 1508-1514, 2000

12 Yamamoto M, "The integrin ligand echistatin prevents bone loss in ovariectomized mice and rats" 139 : 1411-1419, 1998

13 GeonseekRyu, "The Radical Scavenging Effects of Stilbene Glucosides from Polygonum multiflorum" 대한약학회 25 (25): 636-639, 2002

14 Gittens SA, "Systemic bone formation with weekly PTH administration in ovariectomized rats" 7 : 27-37, 2004

15 Yamaguchi K, "Suppressive effect of norzoanthamine hydrochloride on experimental osteoporosis in ovariectomized mice" 22 : 920-924, 1999

16 Chen WS, "Structural elucidation of a new tetrahydroxystilbene of Radix Polygoni Multiflori Preparata and study on its cardiovascular activity" 35 : 906-908, 2000

17 JaeHyun Lee, "Single Subcutaneous Dose Toxicity of Polycan？, a β-Glucan Originated from Aureobasidium in Mice" 한국실험동물학회 21 (21): 299-305, 2005

18 Weinreb M, "Short-term healing kinetics of cortical and cancellous bone osteopenia induced by unloading during the reloading period in young rats" 431 : 449-452, 1997

19 Horcajada-Molteni MN, "Rutin inhibits ovariectomy-induced osteopenia in rats" 15 : 2251-2258, 2000

20 Andersson N, "Repeated in vivo determinations of bone mineral density during parathyroid hormone treatment in ovariectomized mice" 170 : 529-537, 2001

21 Yang PY, "Reduction of atherosclerosis in cholesterolfed rabbits and decrease of expressions of intracellular adhesion molecule-1 and vascular endothelial growth factor in foam cells by a water-soluble fraction of Polygonum multiflorum" 99 : 294-300, 2005

22 Chen K, "Recent advances in studies on traditional Chinese anti-aging material medication" 13 : 223-226, 1993

23 Li M, "Protective effect of polygonum multiflorum thunb on the cerebral cholinergic neurofibers in rats" 28 : 361-364, 2003

24 Chan YC, "Polygonum multiflorum extracts improve cognitive performance in senescence accelerated mice" 31 : 171-179, 2003

25 Lin JT, "Nonmedical management of osteoporosis" 14 : 441-446, 2002

26 Yim Tk, "Myocardial protective effect of an anthraquinone-containing extract of Polygonum multiflorum ex vivo" 64 : 607-611, 1998

27 Ke HZ, "Long-term treatment of lasofoxifene preserves bone mass and bone strength and dose not adversely affect the uterus in ovariectomized rats" 145 : 1996-2005, 2004

28 Yin J, "In vivo antiosteoporotic activity of a fraction of Dioscorea spongiosa and its constituent, 22-O-methylprotodioscin" 70 : 220-226, 2004

29 Xiao PG, "Immunological aspects of Chinese medicinal plants as antiageing drugs" 38 : 167-175, 1993

30 Alexander JM, "Human parathyroid hormone 1-34 reverses bone loss in ovariectomized mice" 16 : 1665-1673, 2001

31 Li R, "Hormone replacement therapy and breast carcinoma risk in Hispanic and non-Hispanic women" 95 : 960-968, 2002

32 Dourador EB, "Hormonal and biochemical parameters in postmenopausal osteoporosis" 52 : 60-62, 1997

33 Diez F, "Guidelines for the diagnosis of osteoporosis by densitometric methods" 25 : 403-415, 2002

34 Bonaiuti D, "Exercise for preventing and treating osteoporosis in postmenopausal women (Cochrane Review)" CD000333-, 2002

35 Fujioka M, "Equol, a metabolite of daidzein, inhibits bone loss in ovariectomized mice" 134 : 2623-2627, 2004

36 Gowen M, "Emerging therapies for osteoporosis" 5 : 1-43, 2000

37 Sandstedt J, "Elevated levels of growth hormone increase bone mineral content in normal young mice, but not in ovariectomized mice" 137 : 3368-3374, 1996

38 Murakami H, "Effects of tiludronate on bone mass, structure, and turnover at the epiphyseal, primary, and secondary spongiosa in the proximal tibia of growing rats after sciatic neurectomy" 9 : 1355-1364, 1994

39 Pytlik M, "Effects of tibolone on the development of osteopenia induced by ovariectomy in rats" 54 : 35-43, 2002

40 Ladizesky MG, "Effect of melatonin on bone metabolism in ovariectomized rats" 70 : 557-565, 2001

41 Wang W, "Effect of alcoholic extract of prepared radix Polygoni multiflori on preventing atherosclerosis in Japanese quail" 4 : 748-750, 1984

42 Sun D, "Dietary n-3 fatty acids decrease osteoclastogenesis and loss of bone mass in ovariectomized mice" 18 : 1206-1216, 2003

43 Fonseca D, "Daidzein together with high calcium preserve bone mass and biomechanical strength at multiple sites in ovariectomized mice" 35 : 489-497, 2004

44 Joseph, M. L., "Current concepts review osteoporosis" 223-224, 1983

45 Tarvainen R, "Clodronate prevents immobilization osteopenia in rats" 65 : 643-646, 1994

46 Sakai A, "Bone marrow cell development and trabecular bone dynamics after ovariectomy in ddy mice" 23 : 443-451, 1998

47 Syed Z, "Bone densitometry: applications and limitations" 24 : 476-484, 2002

48 Jae Geul Shim, "Bone Loss Preventing Effect of Sophorae Fructus On Ovariectomized Rats" 대한약학회 28 (28): 106-110, 2005

49 Kim HJ, "Bombycis corpus extract prevents amyloid-beta-induced cytotoxicity and protects superoxide dismutase activity in cultured rat astrocytes" 43 : 11-16, 2001

50 Chan YC, "Beneficial effects of different Polygonum multiflorum Thunb. Extracts on memory and hippocampus morphology" 48 : 491-497, 2002

51 Tanaka S, "Acute effects of ovariectomy on wound healing of alveolar bone after maxillary molar extraction in aged rats" 262 : 203-212, 2001

52 Edwards MW, "17 beta estradiol stimulation of endosteal bone formation in the ovariectomized mouse: an animal model for the evaluation of bone-targeted estrogens" 13 : 29-34, 1992