Neuronal Nitric Oxide Synthase-Immunoreactive Neurons In the Hamster Visual Cortex: Lack of Co-localization with Parvalbumin

국문 초록 (Abstract)

산화질소(NO)와 칼슘 결합 단백질은 중추신경계의 다양한 세포들에서 나타나며, 이들은 각각 중요 신호전달 분자와 칼슘 완충 분자이다. 본 연구는 햄스터의 시각피질에서 뇌산화질소 합성효소 (nNOS)와 parvalbumin을 포함하는 뉴런들의 분포와 이들의 co-localization 양상을 면역세포화학적 기법을 이용하여 알아보았다. 햄스터 시각피질에서 parvalbumin에 대한 면역 반응성을 나타내는 뉴런들의 전체 수는 nNOS에 대한 면역 반응성을 보이는 뉴런들의 수보다 17배나 많았다. 가장 큰 차이는 시각피질 제5충에서 발견되었으며, 이곳에서 parvalbumin-면역 반응성 뉴런이 nNOS-면역 반응성 뉴런들의 수보다 54.7배나 높았다. nNOS-또는 parvalbumin-면역 반응성 뉴런들은 크기와 형태, 분포 방식이 시각피질에서 유사하게 나타났다. 그러나 이색 면역형광 기법은 햄스터 시각피질에서 nNOS와 parvalbumin을 모두 발현하는 뉴런은 없음을 보여주었다. 본 연구의 결과는 nNOS와 칼슘 결합 단백질 사이의 co-localization양상이 종간에 차이가 존재함을 나타내며 또한 시각피질에 있는 nNOS-면역 반응성 뉴런들의 다양성과 이질성뿐만 아니라 동물 다양성 이해의 중요성을 함께 제시한다고 볼 수 있다.

다국어 초록 (Multilingual Abstract)

Nitric oxide (NO) and calcium-binding proteins occur in various types of cells in the central nervous system. They are important signaling and calcium buffering molecules, respectively. In the present study, using immunocytochemistry we examined the distribution and the co-localization pattern of neurons containing neuronal nitric oxide synthase (nNOS) and parvalbumin in the visual cortex of hamster. The overall number of parvalbumin-immunoreactive (IR) neurons was 17 times higher than that of the nNOS-IR neurons in the hamster visual cortex. The highest differences were found in layer V, where parvalbumin-IR neurons were 54.7 times more abundant than nNOS-IR neurons. Many nNOS- and parvalbumin-IR neurons were similar in size, shape, and manner of distribution in the visual cortex. However, two-color immunofluorescence revealed that no neurons in the hamster visual cortex expressed both nNOS and parvalbumin. The present results indicate that there are subtle species differences in the co-localization pattern between nNOS and calcium-binding proteins. The present results also suggest not only the heterogeneity and functional diversity of nNOS-IRneurons in the visual cortex, but also the importance of understanding animal diversity

참고문헌 (Reference)

1 "a reliable marker for the effects of monocular deprivation in the rat visual cortex" celleri : 749-51 753, 1992

2 "a gene for a novel calcium-binding protein expressed principally in neuron" 1343-1353, 1990

3 "Transient co-localization of calretinin and calbindin-D28K in developing visual cortex of monkey" 825-837, 1995

4 "Three distinct families of GABAergic neurons in rat visual cortex" 347-358, 1997

5 "The distribution of calcium-binding proteins in the lateral geniculate nucleus and visual cortex of new world monkey" 625-642, 1998

6 "The distribution of NADPH diaphorase and nitric oxide synthase in relation to the functional compartments of areas V1 and V2 of primate visual cortex" (s) : 499-511, 2000

7 "The distribution and morphology of calbindin D28k- calretinin-immunoreactive neurons in the visual cortex of mouse" 143-14 149, 2002

8 "The development of parvalbumin and calbindin-D28K immunoreactive interneurons in kitten visual cortical areas" 1-21, 1994

9 "The chemical heterogeneity of cortical interneurons calbindin and parvalbumin immunoreactivity in the rat" 261-266, 1996

10 "The calcium-binding protein calretinin is localized in a subset of interneurons in the rat cerebral cortex" 93-103, 1993

11 "The S100 family of EF-hand calcium-binding proteins" 134-140, 1996

12 "Subcellular localization of GABA receptor subunits in rat visual cortex" 2001182-197

13 "Role of nitric oxide in the regulation of monoaminergic neurotransmission" 459-466, 2000

14 "Quantitative distribution of parvalbumin and calbindin D28k immunoreactive neurons in the visual cortex of normal and Alzheimer cases" 278-291, 1998

15 "Parvalbumin and calbindin D-28K immunoreactive neurons in area MT of rhesus monkey" 141-149, 2001

16 "Organization and plasticity of GABA neurons and functional aspects" 57-66, 1992

17 "Nitric oxide synthese in rat visual cortex" ushev : 57-67, 2004

18 "Nitric oxide synthase-positive neurons in the rat superior colliculus colocalization of NOS with NMDAR1 glutamate receptor" 501-507, 2001

19 "Nitric oxide synthase in the visual cortex of monocular monkeys as revealed by light and electron microscopic immunocytochemistry" 97-113, 1993

20 "Nitric oxide synthase and calcium-binding protein- containing neurons in the hamster visual cortex" 30-18 39, 2004

21 "Nitric oxide actions in neurochemistry" 97-110, 1996

22 "Neurochemical compartmentation of monkey and human visual cortex similarities and variations in calbindin immunoreactivity across species" 1109-1120, 1993

23 "NADPH-diaphorase histochemistry in the macaque striate cortex" 388-397, 1986

24 "Morphometric multivariate analysis of GABAergic neurons containing calretinin and neuronal nitric oxide synthase in the mouse hippocampus" jin : 2001195-204

25 "Morphological analyses of NADPH-diaphorase/ nitric oxide synthase positive structures in human visual cortex" 770-782, 1994

26 "Molecular basis of plasticity in the visual cortex" 369-378, 2003

27 "Localization of calcium-binding protein parvalbumin-immunoreactive neurons in mouse and hamster visual cortex" 542-9 547, 1999

28 "Immunohistochemical localization of calcium- binding proteins in the adult and developing visual cortex of cats" 563-577, 1987

29 "Immunocytochemical study on the distribution of NOS-immunoreactive neurons in the cerebral cortex of aged rats" 2171-2174, 1998

30 "Immunocytochemical localization of nitric oxide synthase-containing neurons in mouse and rabbit visual cortex and co-localization with calcium- binding proteins" in press -19, 2005

31 "Immunocytochemical localization of calretinin in the superficial layers of the cat superior colliculus" 325-335, 2002

32 "Immunocytochemical localization of calcium-binding protein calretinin containing neurons in cat visual cortex" 721-7 725, 1997

33 "Explorations of the primary visual cortex" 515-299 524, 19821955-1978

34 "Does the visual system of the flying fox resemble that of primates? The distribution of calcium-binding proteins in the primary visual pathway of Pteropus poliocephalus" 73-87, 2000

35 "Distribution of parvalbumin immunoreactivity in the visual cortex of old world monkeys and humans" 417-432, 1990

36 "Differential subcellular localization of forward and feedback interareal inputs to parvalbumin expressing GABAergic neurons in rat visual cortex" 346-360, 1999

37 "Developmental changes in calretinin expression in GABAergic and nonGABAergic neurons in monkey striate cortex" 78-92, 1995

38 "Development of the calcium-binding proteins parvalbumin and calbindin in monkey striate cortex" 626-646, 1991

39 "Development and function of metabotropic glutamate receptors in cat visual cortex" 102-107, 1999

40 "Comparative analysis of calcium-binding protein-immunoreactive neuronal populations in the auditory and visual systems of the bottlenose dolphin" 203-237, 1998

41 "Co-localization of parvalbumin and calbindin D-28k in human cortical and subcortical visual structures" 41-52, 1997

42 "Changes of calretinin calbindin D28K and parvalbumin immunoreactive neurons in the superficial layers of the hamster superior colliculus following monocular enucleation" 104-108, 2002

43 "Calretinin-immunoreactive local circuit neurons in area 17 of the cynomolgus monkey" 113-132, 1997

44 "Calretinin- immunoreactive neurons in the primary visual cortex of dolphin and human" 1992181-188

45 "Calretinin and calbindin D28K immunoreactivity in the superficial layers of the rabbit superior colliculus" 3847-3852, 1998

46 "Calcium-binding proteins in the nervous system" 303-307, 1992

47 "Calcium-binding proteins immunoreactivity in the human subcortical and cortical visual structures" 997-1009, 1996

48 "Calcium-binding proteins and neuropeptides as molecular markers of GABAergic interneurons in the cat visual cortex" 538-544, 1991

49 "A morphological study of neurons expressing NADPH diaphorase activity in the visual cortex of the Golden hamster" e : 221-230, 1996