블루베리 잎 추출물의 항산화 및 항염증 효과 = Anti-oxidant and Anti-inflammatory Effects of Extracts from Blueberry(Vaccinium ashei) Leaf

다국어 초록 (Multilingual Abstract)

This study investigated the anti-oxidative and inhibitory activity of tyrosinase and the anti-inflammatory effects of blueberry leaf extracts for use as a functional ingredient in cosmetic products. Extraction yields via hot water and ethanol were 19.20% and 17.37%, respectively. As a results of measuring anti-oxidation, the electron donating activities of both hot water- and ethanol-extracts increased in a concentration-dependent manner, and showed 65.15% and 91.87% at 500 ㎍/㎖, respectively. The SOD-like activities were higher at the hot water extract. In the measurement of ABTS radical cation decolorization, both water- and ethanol-extracts showed a similar effect. Regarding the inhibitory activity of tyrosinase, the ethanol extract was significantly higher(83.43%) than the hot water extract(28.45%) at 1,000 ㎍/㎖. In these results, the topical application of blueberry leaf extracts to the epidermis ameliorated TPA-induced irritant contact dermatitis. Substantial reductions were observed in skin thickness and tissue weight, neutrophil-mediated myeloperxidase activity, and various histopathological indicators. These results suggest that an ethanol extract of blueberry leaf may act as a natural ingredient for functional cosmetics.

목차 (Table of Contents)

• Abstract
• Ⅰ. 서론
• Ⅱ. 실험방법
• Ⅲ. 실험결과 및 고찰
• Ⅳ. 결론
• 참고문헌

참고문헌 (Reference)

1 모정희, "화장품 소재로서 더덕과 발효더덕 추출물의 피부관련생리활성 비교탐색" 한국인체미용예술학회 14 (14): 49-60, 2013

2 임정대, "한국산 약용식물로부터의 SOD 활성 및 Phenolic Compounds 함량 비교" 한국약용작물학회 12 (12): 191-202, 2004

3 이상옥, "차가버섯의 온도단계별 물추출물의 항산화성 비교" 한국식품영양과학회 34 (34): 139-147, 2005

4 윤미연, "질경이 seed 추출물의 항산화 활성 및 미백작용에 관한 연구" 한국인체미용예술학회 14 (14): 259-269, 2013

5 박주아, "소리쟁이(Rumex crispus L.) 잎 추출물의 항산화 효과 및 각질세포 보호효과" 한국인체미용예술학회 12 (12): 189-198, 2011

6 이세나, "블루베리가 인체 유방암세포 MCF7에서 세포 사멸 관련 유전자 발현에 미치는 영향" 동아시아식생활학회 20 (20): 30-36, 2010

7 정희록, "블루베리 잎의 영양성분 분석 및 항산화 활성" 한국식품저장유통학회 19 (19): 604-610, 2012

8 이준구, "배지의 종류와 조성이 Highbush 블루베리 삽수의 생육 및 발근에 미치는 영향" 한국원예학회 25 (25): 355-359, 2007

9 함대현, "급성염증유발 동물모델에서 蒲公英의 염증억제 효과" 한의병리학회 22 (22): 810-814, 2008

10 문혜경, "국내산과 미국산 블루베리의 이화학적 품질특성" 한국식품저장유통학회 20 (20): 524-531, 2013

11 정창호, "국내 시판 블루베리와 라즈베리의 영양성분 분석 및 항산화 활성" 한국식품영양과학회 37 (37): 1375-1381, 2008

12 Kang, Y.H., "The nitrite scavenging and electron donating ability of phenolic compounds" 28 (28): 232-236, 1996

13 Kim, J.D., "The new technology development strategy of cosmeceutical with use advanced materials resources" 30 : 427-438, 2004

14 Yagi, A., "The effect of tyrosinase inhibition for aloe" 2981 : 517-519, 1986

15 Westwood, M.N., "Temperate-zone pomology" Timber Press 100-101, 1993

16 Pellegrin, N., "Screening of diatry carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2,2'-azinobis(3-ethylbenzo- thiazoline-6-sulfonic acid) radical cation decolorization assay" 299 : 379-389, 1998

17 Fresco, P., "New insights on the anti-cancer properties of dietary polyphenols" 26 : 747-766, 2006

18 Bradley, P.P., "Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker" 78 : 206-209, 1982

19 Marklund, S., "Involvement of the superoxide anion radical in the acntioxidant of pyrogallol and a convenient assay for superoxide dismutase" 47 : 468-474, 1974

20 Levine, A., "H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resisitance response" 79 : 583-593, 1994

21 Boo, Y.C., "Effect of tea catechins on the hydroxyl radical induced degradation of DNA components" 1st Scientific Conference of the Asian Soc. Cosm. Sci. Kobe 143-149, 1993

22 Kim, J.D., "Development of cosmetics by using Cacao cholcolate" 4 : 3-10, 2007

23 Lee, K.W., "Cancer chemo-preventive effect and physiological activities of Cacao chocolate" 4 : 27-29, 2007

24 Seeram, N.P., "Blackberry, black raspberry, blueberry, cranberry,m red raspberry, and strawberry extracts inhibit growth and stimulate apoptosis of human cancer cells in vitro" 54 : 9329-9339, 2006

25 Bailey, A.J., "Biological significance of the intermolecular crosslinks of collagen" 251 : 105-109, 1974

26 Correa, H., "Anti-inflammatory effects of the gorgonian Pseudoptero- gorgia elisabethae collected at the Islands of Providencia and San Andrés (SW Caribbean)" 6 : 5-12, 2009