NIH3T3 세포에서 UVB에 의한 세포고사에 미치는 옻 추출물과 fisetin의 효과 = Effect of Rhus verniciflua Stokes Extract and Fisetin on UVB-induced Apoptosis in NIH3T3 Cells

다국어 초록 (Multilingual Abstract)

For a long time Rhus vemiciflua Stokes (RVS) has been traditionally used as a herbal plant in Asia. In this study, we have investigated the effect of acetone extract of Rhus verniciflua Stokes (RVSE) and fisetin, a component of RVSE, on DNA damage response in NIH3T3 cells. Exposure of cells to DVB light $(200 J/m^2)$ and postincubation in growth medium for 48 hr resulted in a decrease of cell viability to about $10-20\%$ of nontreated control. Addition of various concentrations of RVSE in the postincubation medium, however, significantly increased the cell viability as compared with the values expected. The genotoxicity-decreasing effect was also demonstrated in cells exposed to UVB light and incubated in medium containing fisetin. The genotoxicity-decreasing effect of RVSE and fisetin was further demonstrated by various analyses including cell morphology studies, trypan blue exclusion assay and DAPI staining. By Annexin V binding analysis, RVSE and fisetin were shown to decrease the early apoptosis induced by UVB exposure. These results suggest the RVSE contain components that either increase the DNA repair or decrease the apoptosis in UVB-exposed cells.

참고문헌 (Reference)

1 "한국의 자원식물" 서울대학교 출판부 292-294, 1996

2 "p53-mediated regulation of proliferating cell nuclear antigen expression in cells exposed to ionizing radiation" 12-20, 1999

3 "activities of quercetin on normal cells quercetin protects mouse thymocytes from glucose oxidase-mediated apoptosis" 386-397, 2003

4 "Trichothiodystrophy fibroblasts are deficient in the repair of ultraviolet- induced cyclobutane pyrimidine dimers and" 6 526-4 532, 2004

5 "Toxic effects of ultraviolet radiation on the skin" 298-308, 2004

6 "Temporal events in skin injury and the early adaptive responses in ultraviolet-irradiated mouse skin" 201-207, 2000

7 "Suppression of genotoxicity of carcinogens by" 370-376, 1992

8 "Silibinin protects against photocarcinogenesis via modulation of cell cycle regulators" 6349-6356, 2004

9 "Roles of cytochrome P-450 enzymes in chemical cacinogenesis and cancer chemotherapy" 2946-2954, 1989

10 "Respective roles of cyclobutane pyrimidine dimers and minor photoproducts in ultraviolet mutagenesis of repair-deficient xeroderma pigmentosum A cells" 6 1729-4 1735, 2000

11 "Putting checkpoints in perspective" 433-408 439, 2000

12 "Identification of Rhus verniciflua Stokes compounds that exhibit free radical scavenging and anti-apoptotic properties" -1570, 2002181-191

13 "Hypoxia induces p53 through a pathway distinct from most DNA-damaging and stress-inducing agents" carci (carci): 1177-1182, 2003

14 "Free radicals in foods" 201-212, 2002

15 "Flavonoids inhibit cell growth and induce apoptosis in B16 melanoma 4A5 cells" 20001813-1820

16 "Extracts of Caesalpina sappan L potentiate the apoptosis of NIH3T3 cells exposed to MMS" 2002182-187

17 "Epidermal cell kinetics of pig skin in vivo following UVB is enhanced in hyperproliferative skin condition" 43-53, 1998

18 "Effects of Rhus verniciflua stokes on the plasma level of cholesterol and tumor growth in mouse" 169-175, 1999

19 "Diol and triol type ginseng saponins potentiate the apoptosis of NIH3T3 cells exposed to MMS" 2002192-202

20 "Biological activity of urushiol and flavonoids from Lactree" Chonnam National University 1998

21 "Apoptosis and protein expression after focal cerebral ischemia in rat" 301-312, 1997

22 "Antimutagenic potency of chlorophyllin in the Salmonella assay and its correlation with binding constants of mutagen-inhibitors complex" 164-171, 1993

23 "Antigentoxic properties of Cassia tea Mechanism of action and the influence of roasting process" 85-101, 2004

24 "Advances in flavonoids research since 1992" 481-504, 2000