돼지 회충의 Sorbitol Dehydrogenase 에 관하여 = Sorbitol Dehydrogenase Activity of Ascaris Suum

다국어 초록 (Multilingual Abstract)

The distribution of sorbitol dehydrogenase activity was studied in muscle, intestine and ovary of Ascaris strum. The tissues were fractionated by the method of Schneider and Hogeboom and the enzyme activity was measured using Gerlach and Hiby`s procedure. The results obtained were as follows: 1. The enzyme activity intrude preparations of muscle, intestine and ovary was found to be 1,152 units, 2,647 units and 3,366 units per g wet tissue, respectively. 2. Muscle showed the lowest enzyme activity among the tissues tested. Sventy-eight per cent of the activity was present in the cytosolic fraction(912 units/g wet weight). Twenty per cent of the activity (235 units/g wet tissue) was recovered in the mitochondria) fraction. 3. In the intestine, the activity was twice higher than that of muscle and 72% of the total activity was found in cytosolic fraction. 4. Ascaris ovary had the highest enzyme activity and contained 92% of the activity in the cytosolic fraction (3,400 units/g wet tissue).