DNA microarrays are a well-established technology for measuring gene expression levels. The aim in the study was to gain insight into potential over-expressed effect of amyloid precursor protein sw (APPsw) on modulation of genes for Alzheimer's Diseas...
DNA microarrays are a well-established technology for measuring gene expression levels. The aim in the study was to gain insight into potential over-expressed effect of amyloid precursor protein sw (APPsw) on modulation of genes for Alzheimer's Disease (AD). APPsw-transgenic mice, which has been produced by us previously, provide an important resource for identifying differentially expressed genes, since this transgenic line was shown cognitive deficits along with Aβ-42 depositions at 12 months of age. Using cDNA microarray, we compared mRNA levels in 7.4K cDNA clones from postnatal brain of age-matched wild-type mice and Alzheimer's diseased transgenic mice. A total of 44 differentially expressed genes with a 15 up-regulated and with a 29 down-regulated were found in brains from moderatively transgenic mice compared to non-transgenic littermates. Thus, the results allow to the future studies in the functions of each gene, which may be targeted for developing drugs.