일록춘폐흡충(Paragonimus iloktsuenensis) 수용성 항원의 분자량 별 조직 항원성. : 1. SDS-PAGE 상에서 분리된 분자량 16~18kDa의 조직부위 별 항원성. 1. Tissue localities of molecular weight of 16~18kDa on SDS-PAGE = Antigenic tissue localities by molecular weights of watersoluble antigen of Paragonimus ilokteuenensis

다국어 초록 (Multilingual Abstract)

This study was conducted to determine the worm tissue locality and antigenicity of 16~18kDa protein fraction separated from soluble crude antigen of Paragonimus iloktsuenensis. This fraction was obtained by SDS-PAGE, and regarded as the main antigenic protein fraction of D1 antigen seen in the early infection period (between 2~4 weeks of infection). After performing SDS-PAGE with soluble crude antigen, immune antibody (16~18-Ig) was prepared by immunizing gel antigenic emulsion(16~18A) of 16~18kDa protein fraction into a rabbit, and immune responses of worm tissues (tegument, intestinal epithelium, and vitelline gland) were observed using the immunogold labeling method. The results showed that 16~18kDa antigenic protein was present in all the tissues in which this protein was scarcely contained in the tegument, intestinal epithelium and vitelline gland. The antigenicity of intestinal tissue was significantly weak as compared with crude antigen(PIA). Although tissue antigenicity was not strong compared with the amount of protein, we believe that 16~18kDa antigenic protein was the main antigenic protein, being 64% of D1 antigenic protein and that the strong antigenicity of soluble antigen before the development of the vitelline gland(2 weeks before) was decided from this 16~18kDa antigenic protein originated from intestinal tissue.