Dynamic changes of gangliosides expression during thedifferentiation of embryonic and mesenchymal stem cellsinto neural cells

다국어 초록 (Multilingual Abstract)

Stem cells are used for the investigation of developmental processes at both cellular and organism levels and offer tremendous potentials for clinical applications as an unlimited source for transplantation. Gangliosides, sialic acid-conjuga - ted glycosphingolipids, play important regulatory roles in cell proliferation and differentiation. How - ever, their expression patterns in stem cells and during neuronal differentiation are not known. Here, we investigated expression of gangliosides during the growth of mouse embryonic stem cells (mESCs), mesenchymal stem cells (MSCs) and differentiated neuronal cells by using high-performance thin-layer chromatography (HPTLC). Monosialoganglioside 1 (GM1) was expressed in mESCs and MSCs, while GM3 and GD3 were expressed in embryonic bodies.
In the 9-day old differentiated neuronal cells from mESCs cells and MSCs, GM1 and GT1b were exp - ressed. Results from immunostaining were consis - tent with those observed by HPTLC assay. These suggest that gangliosides are specifically expressed according to differentiation of mESCs and MSCs into neuronal cells and expressional difference of ga - ngliosides may be a useful marker to identify differentiation of mESCs and MSCs into neuronal cells.

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