광학영상시스템을 이용한 악성흑색종luc 동물모델의 생체발광영상 정량적 분석 = Quantitative analysis of bioluminescence imaging of Malignant melanoma with luciferase in mouse models using optical imaging system

다국어 초록 (Multilingual Abstract)

ABSTRACT

Quantitative analysis of bioluminescence imaging of Malignant melanoma with luciferase in mouse models using optical imaging system

Jae Young, Kim

Major in Biomedical Engineering, Department of Medical Science,
Graduate School, Korea University, Seoul, Korea
(Director : Prof. Chil Hwan Oh, M.D., Ph.D.)

Background/Purpose: Annually, incidence of malignant melanoma in asian has been increased as western people had been done. This cancer needs a lot of researches for early diagnosis and treatment. The purpose of this study is to analyze quantitatively in mouse melanoma model of subcutaneous melanoma for using preclinical evaluation of new management methods.
Nowadays, non-invasive evaluation of small animal tumor models could be used with Micro Magnetic Resonance Imaging (MRI), Micro Computed Tomography (CT), Single Photon Emission Computed Tomography (SPECT), Micro Positron Emission Tomography (PET). These devices are very expensive to be used and it takes long time to obtain the image of cancer model using these devices.
However, the bioluminescence and fluorescence imaging system is much cheaper than others. Also it takes short time to acquire image. The bioluminescence and fluorescence imaging is a widely used non-invasive method for sensing gene expression, protein functions and other biological processes in animal models.
Methods: All experiments were performed using BALB/c nude inbred mice in accordance to Institutional Review Board (IRB) standards and procedure protocols of Korea University Medical Center (KUMC). Subcutaneous xenografts were established by injection of 5×106 B16-F1 cell line that had been transduced lentiviral vector containing luciferase. In vitro and in vivo bioluminescence images were obtained using homemade system.
In our imaging system, the cooling Charge-Coupled Device (CCD) camera (Princeton Instruments PIXIS, Roper Scientific, Trenton, NJ) was used for data acquisition. Acquired images were analyzed using MatlabTM software (The mathworks, Inc., MA, U.S.A.).
Results: Bioluminescence imaging could be used for evaluation of degree of subcutaneous xenograft tumor models (melanoma B16-F1 cell line that tranceduced lentiviral vector containing firefly luciferase). Bioluminescent signal had increased according to tumor size.
Bioluminescence imaging is supposed to be a powerful tools for in vivo evaluation of mouse model of xenograft melanoma. This technology is a cost effective and has high sensitivity. So, our bioluminescence imaging system can be use for preclinical study for new therapeutic agent against to malignant melanoma.

목차 (Table of Contents)

• Ⅰ. 서 론 1
• Ⅱ. 연구방법 5
• 1. 악성흑색종 세포 및 동물모델 구축방법 5
• 2. 광학 분자영상 시스템 구축방법 8
• Ⅲ. 연구결과 18
• Ⅳ. 고 찰 36
• Ⅴ. 결 론 41
• Ⅵ. 참고문헌 43