Attachment and Growth of Bovine Aortic Endothelial Cells on Amine Surfaces Formed by Using Plasma-Enhanced Chemical-Vapor Deposition

다국어 초록 (Multilingual Abstract)

Cells attached on surfaces can be used for many biological applications, such as cell chips. For some purposes, surfaces with excellent cell attachment and subsequent growth are needed. In this work, plasma-polymerized ethylenediamine (PPEDA) thin films were deposited on glass slides by using a plasma-enhanced chemical-vapor deposition system with an ethylenediamine precursor. In previous reports, PPEDA thin films were shown to have a high density of amine functional groups on their surfaces. Bovine aortic endothelial cells (BAECs) tested in this study were grown on a
PPEDA film in Dulbecco's modified Eagle's medium containing 20 % Fetal Bovine Serum at 37 ℃ and 5 % CO2. The BAECs were revealed to have adhered to the surface of the PPEDA film and to have grown as on commercial culture dishes. By estimating the cell growth rates, we found the PPEDA film to be better than commercial culture dishes. The PPEDA film can be applied for a variety of biological assay techniques.

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