원보 ; 흰쥐의 소장에서 음식물이 암피실린의 흡수에 미치는 영향 = Original Articles ; Effect of Food on Ampicillin Absorption in the Rat Intestine

다국어 초록 (Multilingual Abstract)

The purpose of this study is to verify the interaction between food and ampicillin which is one of the aminopenicillins known to be absorbed by a specified dipeptide transporter in the small intestine. The absorption of ampicillin was measured in the presence of the high carbohydrate food, high fat food, and high protein food, and compared with that in the presence of the control normal food. In situ single-pass perfusion method was chosen in these experiments using two jejunal segments in the rat. Reduction in the absorption of ampicillin was not shown, when both high carbohydrate food and high fat food were co-perfused with ampicillin. When the high protein food was co-perfused with ampicillin, the difference of C_(out)/C_(in) of ampicillin ratio was 0.084 ± 0.082. showing a trend of reduced absorption without a significance. Further, glyclysarcosine (Gly-Sar) which is a stable dipeptide in the small intestine was used in order to see the direct competitive inhibition with ampicillin on the dipeptide transporter. The difference of C_(out)/C_(in) ratio was 0.078 ± 0.020 in the presence of 10 mM Gly-Sar, showing a significant inhibition of ampicillin absorption (p<0.02). It suggests that dietary di- and tripeptides, the digestive products of protein food, might have influence on the absorption of ampicilln, and that ampicillin could be given at the fasting state for better absorption.