제주도 한라산에 서식하는 도입종 야생멧돼지에 대한 분자유전학적 분석 = A Molecular Genetic Analysis of the Introduced Wild Boar Species (Sus scrofa coreanus) on Mount Halla, Jeju Island, Korea

다국어 초록 (Multilingual Abstract)

An wild boar species which has been known as an extinct species on Jeju Island, was recently observed in the surrounding areas of Mount Halla. Based on the molecular techniques, this study examines whether they are crossbred with domesticated pig breeds. Intraspecific genetic relationships with other wild boar populations and molecular sexing were examined as well. Total of four molecular markers on mitochondrial DNA(control region and ND2) and nuclear DNA(MC1R and KIT) were applied to test crossbreeding between with domesticated pig breeds, such as Landrace, Large White, Berkshire, Hampshire, and Duroc. All individuals of wild boar population had identical mtDNA control region(CR) sequences. In addition, the sequences were the same as those of some native pig breeds which are distributed in Northeast China, but different from those previously reported from the Korean Peninsula up to date. These results suggest that this population may have originated from a genetic lineage had been not previously studied and genetically related to Chinese native pig breeds. Molecular sexing results show that there are twice as many females as male. Thus the population is under expansion and its size will dynamically increase if not controlled.

국문 초록 (Abstract)

제주도에서는 절멸된 것으로 간주되었던 멧돼지들이 최근 한라산 인근지역에서 발견되었다. 본 연구는 분자유전학적 실험기법을 바탕으로 한라산 멧돼지들이 가축돼지들과 이종교배된 것들인지를 조사하였다. 또한 동일 종내에서의 유전적 유연관계와 분자 성판별을 시험하였다. 가축돼지 품종들(Landrace, Large White, Berkshire, Hampshire, Duroc)과의 교배여부는 핵 DNA와 미토콘드리아 DNA에서 4 종류의 분자 표지인자(MC1R, KIT, 조절영역, ND2)를 적용하여 시험하였다. 야생멧돼지 집단의 모든 개체들이 동일한 mtDNA 조절영역 서열을 나타내었고, 그 서열들은 중국 동북부 재래돼지들과 동일하였으나 기존에 보고된 한반도 멧돼지의 서열들과는 다른 것으로 확인되었다. 이상의 연구결과는 한라산 멧돼지집단이 중국 재래돼지 품종들과 근연이면서, 기존에 연구되지 않았던 유전적 계통에서 유래한 것으로 사료된다. 분자 성판별 결과 수컷에 비해 암컷이 2 배 이상으로 확인되어, 한라산 야생멧돼지 집단이 팽창하고 있으며, 조절하지 않으면 집단 규모는 극적으로 증가할 것이다.

참고문헌 (Reference)

1 조인철, "한반도 멧돼지 KIT 유전자의 유전적 변이와 신규 돌연변이" 한국동물자원과학회 48 (48): 1-8, 2006

2 오홍식, "한라산 국립공원의 포유류 현황" 한국환경생태학회 21 (21): 235-242, 2007

3 신태균, "제주도 종달리패총유적 4지구에서 출토된 동물뼈 유물의 비교해부학적 관찰" 대한수의학회 42 (42): 309-320, 2002

4 김재환, "돼지 mtDNA D-loop 지역의 Large White 특이 중복현상 탐지" 한국생명과학회 19 (19): 467-471, 2009

5 조인철, "돼지 SRY와 ZF 유전자를 이용한 성판별 기법" 한국동물자원과학회 47 (47): 317-324, 2005

6 조인철, "돼지 Melanocortin Receptor 1(MC1R) 대립유전자 3의 신규 유전변이 탐색" 한국동물자원과학회 46 (46): 1-6, 2004

7 한상현, "돼지 5품종에 있어서 mtDNA ND2 유전자의 선택적 개시코돈의 특성과 빈도" 한국동물자원과학회 46 (46): 903-908, 2004

8 서보영, "대한민국내 주요 돼지 품종의 순종 식별을 위한 품종특이 DNA marker의 활용" 한국동물자원과학회 46 (46): 735-742, 2004

9 Zhao, X., "Zhejiang University, Zhejiang, China. (unpublished data)"

10 Pielberg, G., "Unexpectedly high allelic diversity at the KIT locus causing dominant white color in the domestic pig" 160 (160): 305-311, 2002

11 Oh,H.J, "The studies on the genetic marker of Korean wild pig (Sus coreanus) using RAPD method" 41 (41): 629-636, 1999

12 In-Cheol Cho, "The Robust Phylogeny of Korean Wild Boar (Sus scrofa coreanus) Using Partial D-Loop Sequence of mtDNA" 한국분자세포생물학회 28 (28): 423-430, 2009

13 Ruvinsky, A, "Systematics and evolution of the pig, In Handbook of The Genetics of the Pig" CAB International 1-16, 1998

14 Skogland,T, "Sex ratio in relation to maternal condition and parental investment in wild reindeer Rangifer t. tarandus" 43 : 417-419, 1986

15 Han, S.H., "SINE-like sequence insertion on zinc finger-Y gene and sexual dimorphism in wild boars" GARUDA SCIENTIFIC PUBLICATIONS 32 (32): 165-168, 2007

16 Johansson Moller M., "Pigs with the dominant white coat color phenotype carry a duplication of the KIT gene encoding the mast/stem cell growth factor receptor" 7 (7): 822-830, 1996

17 Epstein,H, "Pig. In: I.L. Mason(eds), Handbook of evolution of domesticated animals" Longman 145-162, 1984

18 Larson, G., "Patterns of East Asian pig domestication, migration, and turnover revealed by modern and ancient DNA" 107 (107): 7686-7691, 2010

19 Andersson, L., "Melanocortin receptor variants with phenotypic effects in horse, pig, and chicken" 994 : 313-318, 2003

20 Kijas J.M., "Melanocortin receptor 1 (MC1R) mutations and coat color in pigs" 150 (150): 1177-1185, 1998

21 Jeju-do and Hallasan-Saengtaemunhwayeonguso, "List of animals and Plants in Mount Halla. Gak, Jeju" 349-, 2006

22 Oh, H.S, "Jeju National University, Jeju, Jeju-do. (personal communication)"

23 Oh, J.G, "Institute of Environmental Resource Research, Jeju, Jeju-do. (unpublished data)"

24 Xu, G.L., "Genetic analysis of the KIT and MC1R genes in Chinese indigenous pigs with belt-like coat color phenotypes" 37 (37): 518-519, 2006

25 Kim, H.C., "Comparative anatomical study of animal bones excavated from the Konaeli archaeological site on Jeju Island" Cheju Nat'l. Univ. 18 : 129-141, 2002

26 Thompson, J.D., "CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting position specific gap penalties and weight matrix choice" 22 : 4673-4680, 1994

27 Shin, T.K., "Archaeological study of animal bones excavated from Cheju Kimnyungri cave site" 36 : 757-761, 1996

28 김재환, "Ancient Pigs on Jeju Island, Korea: Molecular Identification andPhylogenetic Relationship with Extant Native Pigs" 한국유전학회 28 (28): 385-393, 2006

29 Shi, K.R., "Analysis of the MC1R, KIT and ASIP loci in Chinese and European pigs" 37 (37): 300-302, 2006

30 D'Alessandro E., "Analysis of the MC1R gene in the Nero Siciliano pig breed and usefulness of this locus for breed traceability" 31 (31): 389-392, 2007

31 Giuffra, E., "A large duplication associated with dominant white color in pigs originated by homologous recombination between LINE elements flanking KIT" 13 (13): 569-577, 2002