Two strains of Lactobacillus acidophilus NRRL B-2178 and Lactobacillus plantarum ATCC 8014 were treated with N-methyl-N-nitro-N-nitrosoguanidine(NTG). In case of Lactobacillus acidophilus NRRL B-2178, 71 possible mutants were isolated. There was consi...
Two strains of Lactobacillus acidophilus NRRL B-2178 and Lactobacillus plantarum ATCC 8014 were treated with N-methyl-N-nitro-N-nitrosoguanidine(NTG). In case of Lactobacillus acidophilus NRRL B-2178, 71 possible mutants were isolated. There was considerable variation among mutants in acid production and relative amounts of volatile compounds of soy milk prepared from soy protein isolate. LaHA-6 which was excellent in acid production and relative amounts of volatile compounds was found to possess stable characters after 20 daily transfer in MRS broth. In case of Lactobacillus plantarum ATCC 8014, 44 possible mutants were isolated. There was considerable variation among mutants in acid and CO_2 production of modified Elliker's broth medium and MD broth medium, respectively. LpLA-10 which produced lower amounts of acid and higher amounts of CO_2 was found to possess stable characters after 20 daily transfer in MRS broth.