Steroid Receptor Activator modulates proliferation and invasion in the human ovarian cancer cell = Steroid Receptor Activator modulates proliferation and invasion in the human ovarian cancer cell

다국어 초록 (Multilingual Abstract)

Long non-coding RNAs (lncRNAs) are newly identified regulators in tumorigenesis and tumor progression. Although the functions of lncRNAs and the mechanisms regulating their expression are largely unknown, recent studies are beginning to solve thier importance in human health and diseases. Steroid receptor RNA activator (SRA) RNA levels might affect some biological functions, such as proliferation, apoptosis, steroidogenesis, and myogenesis, has been reported. This study investigated the molecular aggressive function of SRA in ovarian cancer. To investigate the role of SRA in the development of ovarian cancer, we examined SRA expression in ovarian cancer tissues (n=14) and corresponding normal tissues (n=16) by real-time polymerase chain reaction. Knockdown of SRA (siSRA) by RNA interference was performed to explore its roles in cell proliferation, migration and invasion assay. SRA expression was significantly upregulated in ovarian cancer tissues compared to normal ovarian tissues. Real-time PCR results showed high expression levels of SRA in SKOV3, TOV112D and OVCA429 human ovarian cancer cell lines. Knockdown of SRA reduced cell proliferation, migration, and invasion in OVCA429 cells. Moreover, SRA knockdown decreased the expression of vascular endothelial growth factor and matrix metalloproteinase-9, which are important for cell motility and metastasis. Therefore, SRA may promote tumor aggressiveness through the upregulation of VEGF and MMP-9. These results identified an important role of SRA in the molecular etiology of ovarian cancer and implicated the potential application of SRA in ovarian cancer therapy.