가시오가피와 더덕 추출물을 첨가한 발효유가 마우스의 면역기능에 미치는 영향

국문 초록 (Abstract)

가시오가피의 변역활성 증진 효과를 알아보기 위하여 선발된 수컷 마우스를 대상으로 하여 발효유에 가시오가피와 더덕 열수 추출건조물(가시오가피 : 더덕 = 8:2)을 혼합하여 투여한 그룹 1㎎/㎖(A), 3 ㎎/㎖(B), 9 ㎎/㎖(C)의 3그룹으로 나누어 임상적용 경로인 경구투여를 선택하여 발효유를 각각 3 ㎖/㎏씩 위내로 직접 투여하였다. 대조군은 발효유만 먹인 그룹(D)과 식염수만 먹인 그룹(E)을 두었다. 오가피의 함량이 ICR계 수컷 마우스의 면역기능에 미치는 영향에 대해 알아본 결과 모든 군에서 마우스의 증체량과 체중 증가는 유의성 있는 차이를 보이지 않았고, 7주와 10주령에 안락사 시킨 마우스의 각 장기의 무게에서도 유의성 있는 차이를 보이지 않았다. 비장계수는 7주령에서는 B군이, 10주령에는 C군이 유의성 있는 증가가 관찰되었다(p＜0.05). 항체생산세포수는 식이급여 7주에 B와 C군이, 10주에 C군이 각각 대조군에 비해 통계적인 유의성이 있었다(p＜0.05). 양적혈구에 대한 응집소가는 식이의 급여기간이 길어질수록 감소하였는데, 7주차에는 A, B, C군에서, 10주차에서는 B와 C군에서 유의성이 있었다(p＜0.05). 혈중 면역글로불린(IgG)의 수치는 7주에서는 C군이, 10주에서는 B와 C군이 유의성이 있었다. 림프구는 B와 C군이 대조군보다 증가하였고, 비장 및 흉선조직에서도 B와 C군이 활발한 면역반응을 보였다. 이상의 결과 가시오가피와 더덕 열수추출건조물이 생체 내에서 면역력을 증강시킬 수 있을 것으로 사료되었다.

다국어 초록 (Multilingual Abstract)

We investigated the immunomodulatory actions of water extract from Acanthopanax senticosus in male ICR mice. The mice were treated with fermented milk containing three added doses of freeze dried extract: 3 ㎎/㎏ (A), 9 ㎎/㎏ (B), and 27 ㎎/㎏ (C) of body weight with Acanthopanax senticosus: Codonopsis lanceolata (8:2) for 7 and 10 weeks, respectively. Organ weights, plaque-forming cell tests, agglutination tests, IgG tests, differential white cell counts, and histological tests were performed at the 7th and 10th weeks of dietary treatment. There were no significant differences in body weight and organ weight. The spleen indices of group B at 7 weeks and group C at 10 weeks were significantly higher than those of the control group (p ＜ 0.05). For the plaque-forming cell test, groups B and C at 7 weeks, and group C at 10 weeks, showed significant increases over the control group (p ＜ 0.05). The agglutination test decreased with an extended experimental period. Groups A, B, and C at 7 weeks, and groups B and C at 10 weeks, had greater antibody responses to sheep red blood cells (SRBC) than the control group. The IgG antibody production of group C at 7 weeks and groups B and C at 10 weeks were significantly higher than the control group (p ＜ 0.05). In groups B and C, lymphocyte percentage was higher than the control group, and their spleen and thymus tissues showed active immune reactions.

목차 (Table of Contents)

• Abstract
• 서론
• 재료 및 방법
• 결과 및 고찰
• 요약
• 문헌

참고문헌 (Reference)

1 Gilbert AB, "prostaglandinsynthesis and humoral immunity in lewis rats" 1187-1194, 1983

2 Tashev TA, "and ultrastructuralstudies on the liver offasted rats" stefa (stefa): 597-20 603, 1976

3 Khim, "Triterpenoidal glycosides of Eleutherococcussenticosus leves" 1618-622, 1971

4 Roy LW, "The retardation of aging in miceby dietary restriction" 641-654, 1986

5 Borkowski B, "The preparation of vegetableproducts containing isofraxidin and Glaucium alkaloidsand evaluation of their choloretic action" 271-283, 1973

6 Szolomicki S, "The influence of active components of Eleutherococcus senticosus on cellular defence and physical fitness in man" 14 : 30-35, 2000

7 Mary L, "The effects of quality andquantity of dietary fat on the immune system" 1983

8 Gaffney BT, "The effects of Eleutherococcus senticosus and Panax ginseng on steroidal hormone indices of stress and lymphocyte subset numbers in endurance athletes" 14 : 431-442, 2001

9 Kim WK, "The effect of sesame oil perilla oil and beeftallow on body lipid metabolism and immune response" 42-53, 1989

10 Richard WL, "The effect of restricteddietary intake on the antibody mediated response of the zinc deficientAlJ mouse" 881-887, 1978

11 Lee KT, "Structure of a new echinocystic acid bisdesmoside isolated from Codonopsis lanceolata roots and the cytotoxic activity of prosapogenins." 50 : 4190-4193, 2002

12 Ha TY, "Separation of subpopulation with suppressor activity" The thymus suppressor cell 13-23, 1974

13 Doherty NS, "Selective effect of immunosuppressive agentsagainst the delayed hypersensitivity response and humoralresponse to sheep red blood cells in mice" 237-242, 1981

14 Hafeez BB, "Protective effect of Cassia occidentalis L. on cyclophosphamide-induced suppression of humoral immunity in mice" 75 : 13-18, 2001

15 Whang WK, "Physiological activities of mixed extracts of Acantopanax senticosus Radicis Cortex and Eucommiae Cortex" 27 : 65-74, 1996

16 Todorovv IN, "Pharmacokinetics and mechanism of actionof glycosides of Eleutherococci Effects of extract on the metabolismand biosynthesis of protein in several organs and tissues ofrats" 529-533, 1984

17 Kim JH, "Pharmacognostcal studies on Codonopsis lanceolata" 14 : 529-534, 1999

18 Ann, "New substances of plant originwhich increase nonspecific resistance" 419-430, 1969

19 Kent LE, "Lack of an influence of dietary fat onmurine natural killer cell activity" 1311-1317, 1989

20 Suh JS, "Isolation of active components on immunocytes from Codonopsis lanceolata" 31 : 1076-1081, 1998

21 Meade CJ, "Importance of the spleen for theimmuno inhibitory action of linoleic acid in mice" 469-473, 1977

22 Lee JH, "Immunostimulative effect of hot water extract from Codonopsis lanceolata on lymphocyte and clonal macrophage" 34 : 732-736, 2002

23 Hirata F, "Hypocholesterolemic effect of sesame ligan in humans." 122 : 135-136, 1996

24 Lee HK, "Human seminal plasma suppresses to intravaginallydeposited sheep red blood cells and sperm" Separation ofimmunosuppressive factors 1989

25 Ferguson PW, "Effects of Eleutherococcussenticosus extracts on hexobarbital metabolism in vivo and invitro" eth (eth): 235-241, 1984

26 Yoon BT, "Effect of hot water extract from Acanthopanax senticosus on systemic anaphylaxis" 34 : 518-523, 2002

27 Asano K, "Effect of Eleutheroccocus senticosusextract on human physical working capacity" 175-177, 1986

28 Han EO, "Effect of Codonopsis lanceolata water extract on the level of lipid in rats fed high diet" 27 : 940-944, 1998

29 Han EG, "Effect of Codonopsis lanceolata water extract on the activities of antioxidative enzymes in carbon tetrachloride treated rats" 26 : 1181-1186, 1997

30 Deyama T, "Constituents and pharmacological effects of Eucommia and Siberian ginseng" 22 : 1057-1070, 2001

31 Chung MS, "Composition and color of Codonopsis lanceolata affected by cultivation methods" 14 : 529534-, 1999

32 Zimmermann T, "Biochemical and substructuralstudies on hepatic and serum lipoprotein metabolism afteracute liver injury induced by thioacetamide in rats" 225-233, 1985

33 Glatthaar-Saalmuller B, "Antiviral activity of an extract drived from roots of Eleutherococcus senticosus" 50 : 223-228, 2001

34 Maeng YS, "Antioxidant activity of ethanol extract fromDeodeok" codo (codo): 311-316, 1991

35 SoutheastAsian I, "An evaluation of some biochemical methodsused in the assessment of protein nutritional status" 262-266, 1972