감비수 향 흡입이 절식을 유도한 쥐의 시상하부에서 섭식관련 신경전달물질의 발현과 인지력에 미치는 영향

국문 초록 (Abstract)

본 연구는 절식을 유도한 ICR계 생쥐에서 감비수 향 흡입이 시상하부에서 leptin과 neuropeptide Y (NPY)의 발현에 미치는 영향과 단기 기억력에 미치는 효과를 알아보고자 하였다. ICR 계열 수컷 쥐 24마리를 무작위 추출법에 의해 대조군, 절식군, 절식과 감비수 향 흡입군(각 군 n=8)의 3군으로 분류하였다. 실험결과, 절식은 시상하부에서 leptin의 발현을 감소시켰고 NPY의 발현은 증가시켰다. 또한, 절식에 의하여 단기 기억력과 해마에서의 신경성장인자인 BDNF의 발현이 감소되었다. 반면에, 절식 중 감비수 향을 흡입 시 시상하부에서 leptin의 발현은 증가되었고 NPY의 발현은 감소되었다. 그리고 감비수 향의 흡입에 의하여 단기 기억력이 향상 되었고 해마에서의 신경성장인자인 BDNF의 발현이 증가되었다. 본 실험 결과, 감비수 향 흡입은 절식 시 증가된 음식섭취에 대한 욕구를 억제할 수 있으며 절식에 의하여 감소된 단기 기억력을 향상시키는 효과가 있음을 알 수 있었다.

다국어 초록 (Multilingual Abstract)

In the present study, we investigated the effects of Gambisu inhalation on the expressions of neurotransmitters implicated in the food intake in the hypothalamus and cognition using starved mice. For this, the effects of Gambisu inhalation on the expressions of leptin and neuropeptide Y (NPY) in the paraventricular nucleus (PVN) of hypothalamus of starved mice were evaluated by immunohistochemistry. Short-term memory of the mice was also assessed using a passive avoidance task and brain-derived neurothrophic factor (BDNF) expression in the hippocampus was determined by Western blot analysis. ICR mice were divided into three groups: control group, starvation group, and starvation with Gambisu inhalation group. The mice in the food-deprivation group were restricted food for 48 h. The mice in the Gambisu inhalation group were exposed to inhale Gambisu for 60 min twice a day for 2 consecutive days. The present results showed that food-deprivation decreased leptin expression and increased NPY expression in the PVN of the hypothalamus. Food-deprivation decreased short-term memory ability and also suppressed BDNF expression in the hippocampus. However, Gambisu inhalation increased leptin expression and decreased NPY expression in the PVN of hypothalamus under the starvation conditions. Gambisu inhalation alleviated starvation-induced short-term memory impairment and enhanced BDNF expression in the hippocampus under the starvation conditions. The present study revealed that Gambisu inhalation might suppress desire for food intake and exert beneficial effect on cognition by enhancing BDNF expression in the hippocampus under the starvation conditions. (Korean J Str Res 2010;18:209∼217)

목차 (Table of Contents)

• 서론
• 재료 및 방법
• 결과
• 고찰
• 참고문헌
• 국문초록

참고문헌 (Reference)

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