병원성 대장균의 이열성독소 검사를 위한 V-ET RPLA법과 ELISA법의 비교 및 혈청균과 독소생성의 관련성 연구 = Comparison of V-ET RPLA and ELISA techniques for the detection of heat-labile enterotoxin of Escherichia coli and the association of serogroup and the toxin production

다국어 초록 (Multilingual Abstract)

The V-ET RPLA and ELISA techniques were compared for the dection of heat-labile enterotoxin of Escherichia coli.
E.coli strains, 1,568 in number, were isolated from 2,617 stool specimens collected from pediatric patients attending 7 clinics or hospitals in Seoul. Among the isolates, 95(6.1%) were either enterotoxigenic, enteroinvasive or
enteropathogenic E.coli serologically. The determined results by Vibrio cholerae E.coli enterotoxin reversed passive latex agglutination (V-ETRPLA) and enzyme-linked immunosorbent assay(ELISA) were as follows.:
1.The detection rate of heat-labile enterotoxin by V-ET RPLA and ELISA were 9.5 per cent and 20.0 per cent respectively, however these two rates showed no signficant difference(p＞0.05).
2.In order to compare the power of detecting heat-labile enterotoxin, the same 55 E.coli isolates were tested by V-ET RPLA and ELISA method.
ELISA showed the detection rate of 20.0 per cent than the rate of 16.4
per cent by V-ET RPLA, but there was no significant difference between V-ET RPLA, but there was no significant difference between V-ET RPLA and ELISA methods(p＞0.05).
3.The size of the proportion of strains producing heat-labile enterotoxin in enterotoxigenic E.cdi(ETEC) Compared to enteropathogenic E.coli(EPEC) indicate no relationship(p＞0.05).

목차 (Table of Contents)

• Ⅰ.緖論
• Ⅱ.硏究資料 및 分析方法
• Ⅲ.結果
• Ⅳ.考察
• Ⅴ.結論
• 〈參考文獻〉
• 〈ABSTRACT〉