녹차 및 천연산물로부터 장염 비브리오균(Vibrio. parahaemolyticus)의 제어를 위한 고추장의 개발 = Development of GOCHUJANG for Controlling V. parahaemolyticus with Green Tea and Natural Products

국문 초록 (Abstract)

V. pnrahaemolyticus를 저해하는 천연산물 조합 추출물을 이용하여 고추장을 제조하였다. 각각의 천연산물 및 천연산물 조합 추출물의 항산화력 크기는 ORP system value를 이용하여 측정하였고, 천연산물 조합 추출물을 이용하여 제조한 고추장을 disk 법으로 IZD (Inhibition zone divided by diameter of disk)를 측정하여 제어의 정도를 비교하였다. 그 결과 한 가지 천연산물에서는 황백, 오미자, 녹차가 높게 나타났고, 이들 천연산물을 조합했을 때도 항산화력이 높게 나타나 기능성 고추장을 제조할 수 있을 것이라 판단하였다. 그리고 항산화력이 높게 측정된 황백, 오미자, 녹차 그리고 황금의 A조합과 A조합에 오가피를 첨가한 B조합의 IZD를 측정하였을 때 각각 $1.57\pm0.0051$과 $1.56\pm0.0071$로 제어력이 뛰어나 A조합과 B조합의 천연산물의 추출물을 이용하여 고추장을 제조하였다 고추장을 제조하였을 때 A조합(IZD: $1076\pm0.0103$)보다 B조합(IZD: $1079\pm0.0103$)이 좋게 나타났고, 이 고추장으로 식초를 첨가하여 초고추장을 제조하였을 때도 A조합(IZD: $2.15\pm0.0071$)보다 B조합(IZD: $2.44\pm0.0086$)이 뛰 어난 제어력을 보였다 여기에 chitosan, 마늘, 메주의 첨가는 유의성 있는 제어력을 나타내지 못하였다.

다국어 초록 (Multilingual Abstract)

Vibriocidal GOCHUJANG was manufactured using extract of natural products and green tea. Antioxidative capacity of natural products and combination of natural products was measured with Oxidation-Reduction Potential (ORP) system value. Inhibitory ability of vibriocidal GOCHUJANG was compared with Inhibition Zone Diameter (IZD) values. Phellodendri cortex, Schizandrae fructus, Theae folium, Scutellaria baicalensis george and Acanthodanacis cortex and combination of these natural products were showed higher antioxidant capacity and inhibitory effect against V. para-haemolyticus. Two kinds of combinations, A combination was composed of Phellodendri cortex, Schizandrae fructus, Theae folium, Scutellaria baicalensis george, and B combination was consisted of Acanthodanacis cortex and A combination, were presented higher inhibition ratio with IZD values as $1.57\pm0.0051$ and $1.56\pm0.0071$, respectively. Vibriocidal GOCHUJANG was prepared based on A and B combination with their higher IZD Value as $1.76\pm0.0103$ and $1.79\pm0.0103$, respectively. When vinegar was added to A and B based GOCHUJANG, their IZD value was more higher as $2.15\pm0.0071$ and $2.44\pm0.0086$, respectively B combination based GOCHUJANG was exhibited higher inhibitory effect than A based GOCHUJANG. Combination of natural products was more important process for increasing vibriocidal effect. But addition of soybean malt or chitosan or a garlic into A or B combined GOCHUJANG, no different effect was exhibited.

참고문헌 (Reference)

1 "radical scavenging activity of flavonoids obtained from some medical plants" (hara and m) : 1 1202-1205, 2001

2 "powerful inhibitors of histamine release induced by anti-immunoglobulin E from rat peritoneal mast cells" ciwujia (ciwujia): 661-662, 1992

3 "as an adaptogen" eth (eth): 345-393, 2000

4 "The growth inhibiting effect of Vibrio parahaemolyticus KCTC 2471 by the natural products bearing antioxidative capacity" 731-739, 2002

5 "The anxiolytic-like effects of Scutellaria baicalensis using elevated plus-maze in rats" 22-27, 2004

6 "Study on the preparation of kochujang with addition of fruit juices" 98-104, 1993

7 "Studies on the evaluation of crude drugs by bioassay" 74-78, 1971

8 "Studies on Scutellariae Radix Effects of various flavonone compounds on lipid peroxidation in rat liver" 1792-1795,

9 "Studies on Scutellariae Radix Anti-arthritic and anti-inflammatory action of methanolic extract and flavonoid components from Scutellariae Radix" higashi : 2411-2415, 1984

10 "Sensory evaluation and changes in physiochemical properties and microflora and enzyme activities of pumpkin-added kochujang" 851-859, 2000

11 "Sensory evaluation and changes in microflora and enzyme activities of red ginseng kochujang" 766-772, 1999

12 "Screening test of antiinflammatory action on the constituents of crude drugs and plants" 782-783, 1970

13 "Quality of Traditional Kochujang Supplemented with Mushrooms" 229-234, 2003

14 "Quality characteristics of kochujang prepared with paecilomyces japonica from Silkworm" 44-49, 2004

15 "Possible mechanism of antidiarrheal effect of berberine" 233-241, 1979

16 "Physiological functions of chitosans as functional food ingredients" Chitin Chitosan 10 55-60, 2005

17 "Physicochemical characteristics of traditional kochujang prepared with various raw materials" 907-912, 1997

18 "Panax ginseng and Eleutherococcus senticosus may exaggerate an already existing biphasic response to stress via inhibition of enzymes which limit the binding of stress hormones to their receptors" 567-572, 2001

19 "Optimization on organoleptic properties of kochujang with additional of persimmon fruits" 1132-1136, 1998

20 "On the mechanism of vasodilating action of berberine" possible i (possible i): 318-323, 1992

21 "Isolation and identification of antimicrobial compound from Jakyak" 445-452, 2000

22 "Inhibitory effects of various flavonoids on histamine ralease peritonial mast cell in vitro" 2724-2729, 1984

23 "Induction of apoptosis by Acanthopanax senticosus HARMS and its component sesamin in human stomach cancer KATO Ⅲ cells" 1213-1216, 2000

24 "Imipenem- EDTA disk method for differentiation of Metallo-β-Lactamase-producing clinical isolates of Pseudomonas spp" 3798-3801, 2002

25 "Hypotensive principles of Astragalus and Hedysarum roots" hiki : 297-302, 1976

26 "Hypotensive and antiinflammatory effects of Astragalus saponin 1" 333-337, 1984

27 "Green tea consumption and the risk of pancreatic and colorectal cancers" 255-258, 1997

28 "Green tea constituent epigallocatechin- 3-gallate and induction of apoptosis and cell cycle arrest in human carcinoma cells" 19971881-1886

29 "Flavone glycoside in the flowers of Paeonia arborea" 154-156, 1969

30 "Effects of the Biological activities of ethanol extract from korean traditional kochujang added with sea tangle" 1-7, 2002

31 "Effects of Kilkyungjijak-Tang on the respiratory system" Blood pressure and isolated ileum 124-131, 1986

32 "Effect of Lycium chinense fruit on the physicochemical properties of kochujang" 461-469, 2003

33 "Effect of Eleutherococcus senticosus on submaximal and maximal exercise performance" 482-489, 1996

34 "Development of new method for antioxidant capacity with ORP-pH system" 514-518, 2004

35 "Cytotoxic effects of E senticosus aqueous extract against L1210 leukemia cells" 270-272, 1984

36 "Comparison of biological activity according to extracting solvents of four Acanthopanax root bark" 21-28, 2000

37 "Coloured Medicinal Plants of Korea" Academic Publishing Co 431-, 1989

38 "Characteristics of B cell proliferation by polysaccharide fraction of Paeonia Japonica miyabe" 126-134, 2001

39 "Changes in quality characteristics of traditional kochujang prepared with apple and persimmon during fermentation" 575-581, 2000

40 "Changes in physiochemical properties of kochujang by red ginseng addition" 760-765, 1999

41 "Changes in microflora and enzymes activities of traditional kochujang prepared with various raw materials" 901-906, 1997

42 "Biochemical effect of berberine" 1081-1084, 1979

43 "Biliary excretion of metabolites of baicalin and baicalein in rats" 209-211, 1990

44 "Antioxidant and hepatoprotective effects of Acathopanax senticosus" 489-494, 2000

45 "Antimicrobial activity of prunus mume and Schizandra chinenis H-20 extracts and their effects on quality of functional kochujang" 893-897, 2003

46 "Antibacterial Constituents from Scutellariae Radix against Streptococcus mutans OMZ176" 99-103, 1997

47 "A study on the extraction efficiency of berberine in Coptidis rhizoma" 83-91, 1997

48 "A reticuloendothelial systemactivating glycan from roots of Astragalus membranaceus" 63-66, 1992

49 "A clinical review of Astrgalus" Sinclair 338-344, 1998

50 "-epigallocatechin gallate on leukemic blast cells from patients with acute myeloblastic leukemia" 135-142, 1997