병풀과 엽산을 함유한 복합미생물 발효 추출물 및 아세틸글루타민, 니코틴산 아데닌 디뉴클레오티드 인산 복합물의 피부 세포노화 억제, 콜라겐생성 촉진, 세포재생 및 세포이동 촉진, 항염, 각질세포분화 촉진, 멜라닌생성 억제 효능 = Effects of the Complex Containing Centella asiatica - and Folic Acid-Ferment Extracts, Acetyl Glutamine, and Nicotinic Acid Adenine Dinucleotide Phosphate on the Inhibition of Senescence and Melanogenesis, Promotion of Collagen Expression, Cellular Regen

다국어 초록 (Multilingual Abstract)

In this study, we investigated effects of the complex material containing Centella asiatica (C. asiatica )- and folic acidfermented extracts, acetyl glutamine, and nicotinic acid adenine dinucleotide phosphate (NAADP) on the improvement of skin condition and skin cell functions. We confirmed cellular effects of fermented extracts of C. asiatica and folic acid using microorganism mixture (CFFE) and acetyl glutamine- and NAADP-containing CFFE (FAN) on cell viability, reactive oxygen species (ROS) generation, cellular senescence, DNA repair, transcriptional activity of NF-κB, regulation of gene expression, cellular regeneration and migration, cell differentiation, and melanin contents in human dermal fibroblasts (HDFs), HaCaT human keratinocytes, B16F10 mouse melanocytes, and NIH-3T3 mouse embryo fibroblasts. CFFE and FAN showed significant protection effects against cellular damage caused by ultraviolet B (UVB) irradiation or ROS generation. The expression levels of Col1A1 was up-regulated and of MMP1 was down-regulated by treatment of CFFE and FAN in UVB-exposed HDFs. UVB-induced NF-κB activation was significantly suppressed by treatment of CFFE and FAN in NIH-3T3 cells. Also the treatment of CFFE and FAN promoted the differentiation on HaCaT cells. The CFFE and FAN suppressed production of melanin in B16F10 cells by inhibition of expression of MITF and TYR. Especially, it was observed that acetyl glutamine and NAADP increase overall effects of CFFE in earlier time points, implicating that these components initiate and boost cellular signals and positive functions mediated by CFFE in the skin. Taken together, these findings suggest that CFFE and FAN might have excellent efects of the anti-aging, reduction of wrinkle and pigmentation, promotion of cellular regeneration, improvement of epidermal barrier quality, and anti-inflammation on the skin cell.

참고문헌 (Reference)

1 김나미, "홍삼성분이 섬유아세포의 콜라겐 생합성과 MMP-I 활성에 미치는 영향" 고려인삼학회 31 (31): 86-92, 2007

2 윤영민, "자외선(Ultravilet)이 피부 및 피부세포 내 신호전달체계에 미치는 영향" 대한피부미용학회 11 (11): 417-426, 2013

3 김창수, "솔잎 추출물이 진피섬유아세포에서 콜라겐 발현 및 생성에 미치는 영향" 대한피부미용학회 10 (10): 817-822, 2012

4 한정선, "솔잎 발효 추출물의 피부노화억제 효능에 대한 연구" 건국대학교 대학원 2013

5 권구정, "병풀추출물 및 숯가루, 진주가루 함유 세안제의 안면피부 개선효과" 대한피부미용학회 10 (10): 581-586, 2012

6 김영주, "굴의 주요 성분인 Taurine이 Collagen과 MMP 발현 조절에 미치는 영향" 대한피부미용학회 11 (11): 393-397, 2013

7 권유빈, "각질형성세포 분화 및 상처 치유에 대한 초유의 효과" 14 : 45-50, 2007

8 Kim YJ, "entella asiatica extracts modulate hydrogen peroxideinduced senescence in human dermal fibroblasts" 20 : 998-1003, 2011

9 An IS, "Titrated extract of Centella asiatica provides a UVB protective effect by altering microRNA expression profiles in human dermal fibroblasts" 30 : 1194-1202, 2012

10 Berridge MJ, "The versatility and universality of calcium signalling" 1 : 11-21, 2000

11 Nishifuji K, "The stratum corneum: the rampart of the mammalian body" 24 : 60-72, 2013

12 Archambault J, "Stretch and interleukin-1beta induce matrix metalloproteinases in rabbit tendon cells in vitro" 20 : 36-39, 2002

13 전혜숙, "SKH-1 생쥐에서 UVB 조사에 의한 육미지황탕의 광노화 피부 억제에 관한 조직학적 연구" 대한피부미용학회 11 (11): 239-252, 2013

14 Bae JT, "Protective effects of fermented Citrus unshiu peel extract against ultraviolet-A-induced photoageing in human dermal fibrobolasts" 26 : 1851-1856, 2012

15 Galione A, "Physiological roles of NAADP-mediated Ca2+ signaling" 54 : 725-732, 2011

16 Chung JH, "Photoaging in Asians" 19 : 109-121, 2003

17 Gohil KJ, "Pharmacological Review on Centella asiatica : A Potential Herbal Cure-all" 5 : 546-556, 2010

18 Weinstein SJ, "Null association between prostate cancer and serum folate, vitamin B (6), vitamin B (12), and homocysteine" 12 : 1271-1272, 2003

19 Barnes PJ, "Nuclear factor-kappaB: a pivotal transcription factor in chronic inflammatory diseases" 10 : 1066-1071, 1997

20 López-Pedrosa JM, "N-acetyl-L-glutamine, a liquid-stable source of glutamine, partially prevents changes in body weight and on intestinal immunity induced by protein energy malnutrition in pigs" 52 : 650-658, 2007

21 Varani J, "Inhibition of type I procollagen production in photodamage: correlation between presence of high molecular weight collagen fragments and reduced procollagen synthesis" 119 : 122-129, 2002

22 Largo R, "Glucosamine inhibits IL-1beta-induced NFkappaB activation in human osteoarthritic chondrocytes" 11 : 290-298, 2003

23 Tran H, "DNA repair pathway stimulated by the forkhead transcription factor FOXO3a through the Gadd45 protein" 296 : 530-534, 2002

24 Brinkhaus B, "Chemical, pharmacological and clinical profile of the East Asian medical plant Centella asiatica" 7 : 427-448, 2000

25 김영주, "Centella asiatica의 추출조건에 따른 항산화 및 콜라겐 합성 효능" 한국미용학회 16 (16): 829-834, 2010

26 이송정, "Centella asiatica 추출물을 함유한 화장품이 20대 남녀의염증성 여드름 및 홍반개선에 미치는 효과" 한국미용학회 16 (16): 176-184, 2010

27 An IS, "Centella asiatica protects against UVB-induced HaCaT keratinocyte damage through microRNA expression changes" 30 : 1349-1356, 2012

28 Park HY, "Cellular mechanisms regulating human melanogenesis" 66 : 1493-1506, 2009

29 Lee YR, "Brazilin inhibits UVB-induced MMP-1/3 expressions and secretions by suppressing the NF-κB pathway in human dermal fibroblasts" 674 : 80-86, 2012

30 윤영민, "Bifidobacterium longum 추출물의 자외선B에 대한 인간 진피섬유아 세포 보호 효능" 대한피부미용학회 10 (10): 887-891, 2012

31 Tomokiyo A, "Alternation of extracellular matrix remodeling and apoptosis by activation of the aryl hydrocarbon receptor pathway in human periodontal ligament cells" 113 : 3093-3103, 2012

32 Arnaud A, "Absorption of enterally administered N-acetyl-L-glutamine versus glutamine in pigs" 23 : 1303-1312, 2004

33 Tsuzaki M, "ATP modulates load-inducible IL-1beta, COX 2, and MMP-3 gene expression in human tendon cells" 89 : 556-562, 2003