The safety evaluation of hazardous substances in pharmaceutical products and discovery of bioactive natural products has been the representative two axes of our research for the past 10 years. Analytical science and related technologies has clearly be...
The safety evaluation of hazardous substances in pharmaceutical products and discovery of bioactive natural products has been the representative two axes of our research for the past 10 years. Analytical science and related technologies has clearly been the central tools in our researches, but considerations for design and use of analytical methods depend on the nature of projects. Because of toxicity and ubiquitousness, trace chemical residues has been the targets of safety evaluation in pharmaceutical and hygiene products. Quantitative chemical analysis for their contents is the first step for risk assessment study. Since the final assessment results impact on the regulatory action and product quality control standards, analytical methods should be established by considering the factors such as reliability of reference materials, acceptable performance characteristics, and measurement uncertainty estimation. Meanwhile, various separation and analytical technologies contributed to discovery and structural analysis of bioactive natural polysaccharides. Size-exclusion and ion-exchange chromatrography are the major purification tools for target polysaccharides from complex matrix of plant extracts. Physiocochemical properties and structure are assessed using HP-GFC, SEM, FT-IR, and NMR analysis. Of note, despite of absence of commercial reference standards, the monosaccharide composition and their pattern of linkage in the macromolecule can be deduced by cheimcal derivatization and GC-MS based identification and quantitation. This presentation discusses diverse applications of analytical technologies in our research group with representative examples.