Rats의 한탄바이러스 및 서울바이러스에 대한 중화항체 형성 = Formation of Neutralizing Antibodies against Hantaan and Seoul Viruses in Rats

다국어 초록 (Multilingual Abstract)

The production of neutralizing antibodies against Hantaan and Seoul viruses in sera from rats were investigated using plaque reduction neutralization test. Rats were bled at one week interval for 2 months after intramuscular inoculation of Hantaan and Seoul viruses and sera were used for titration of neutralizing antibodies against Hantaan and Seoul virus, respectively by plaque reduction neutralization method employing immunoperoxidase staining in Vero E6 cell cultures. In rats inoculated with Hantaan virus, titers of plaque reduction neutralizing antibody against Hantaan virus increased significantly from 320 at one week to 2,560 at 3-4 weeks and persisted for 60 days. In contrast, neutralizing antibody titers against Seoul virus were much lower than those of Hantaan virus and antibody titer was 160, although it persisted for 2 months. In rats inoculated with Seoul virus, titers of neutralizing antibody against Seoul virus were shown similar values to that of Hantaan virus after Hantaan virus inoculation and maximum titer was 10,240 at 3-5 weeks but neutralizing antibody titers against Hantaan virus were very low and peak value was 160-320. In this study using a rat model, it was observed that neutralizing antibodies against Hantaan and Seoul viruses were specific and significantly different depend on inoculation of virus and it was easy to differentiate Hantaan virus infection from Seoul virus infection. This study has also demonstrated that the formation of cross neutralizing antibody between Hantaan and Seoul virus after inoculation of the virus into rats for the first time.