SLUP+WNM 세포지지체에서 osteoblast cell과 saos-2 cell의 in-vitro 세포 배양 특성 비교

다국어 초록 (Multilingual Abstract)

In this study, a novel technique for dual-pore scaffold fabrication using SLUP (salt leaching using powder) and WNM (wire network molding) was proposed. To fabricated dual-pore scaffold, firstly, PCL with size of 63~100mm and NaCl powders with size of 100~180mm were prepared. Secondly, prepared PCL and NaCl powders were mixed using the stirrer at a certain ratio. Subsequently, stainless-steel needles with size of 500mm were inserted into a designed stainless steel mold. And then, the mixture powders were filled in the stainless-steel mold. Afterword, after the mold was pressurized at 30 MPa for 1 min using a lab-made compressor, the mold was heated to melt PCL powders in an oven at 100℃ for 20 min. Thereafter, after the stainless-steel needles were removed in the stainless-steel mold, the PCL/NaCl structure was separated from the stainless-steel mold. And then, the PCL/NaCl structure was soaked in D.I water for 24 hours using a sonicator to leach out the NaCl particles and dry for 24 hours in the desiccator. Consequently, the remaining PCL structure became the dual-pore scaffold. To analyze surface and cross-section characteristics of scaffolds, scaffolds was observed using SEM. Additionally, cell-culture experiments were performed using CCK-8 assay, ALP and compared with the bio-plotter and SLUP scaffolds.

목차 (Table of Contents)

• Abstract
• 1. Introduction
• 2. Material and Method
• 3. Result and discussion
• 4. Conclusion
• 참고문헌