비전이성 외피단백질 cDNA 도입에 의한 감자 바이러스 Y 저항성 연초 품종 개발 = Development of Potato Virus Y Resistant Tobacco Plants by Introduction of Untranslatable Coat Protein cDNA

다국어 초록 (Multilingual Abstract)

A cDNA fragment, named PVY-CP, encoding coat protein(CP) of potato virus Y (PVY-VN) was obtained by RT-PCR. The PVY-CP cDNA was manipulated to construct an untranslatable form in which three stop codons are introduced near the start codon by site-directed mutagenesis. The untranslatable PVY-CP cDNA was inserted into plant expression vector and transferred to Nicotiana tabacum cv. NC 82 by Agrobacterium-mediated transformation method. The untranslatable PVY-CP cDNA transformed plants were regenerated and PVY resistant transformants were selected based on symptom development after mechanical inoculation. Genomic PCR and Southern blot analysis confirmed that one of more copies of the untranslatable PVY-CP cDNA was integrated into the genome of tobacco plants which are highly resistant to PVY.