Polyacrylamide gel isoelectric focusing was used to analyze the grain esterase isozymes of rice varieties, Tongil, Tongilchal and their related parents. Six major bands were revealed in the gel part of high pH and observed different banding patterns. ...
Polyacrylamide gel isoelectric focusing was used to analyze the grain esterase isozymes of rice varieties, Tongil, Tongilchal and their related parents. Six major bands were revealed in the gel part of high pH and observed different banding patterns. Esterase isozyme banding patterns in grains were separated into three types, namely Est-Ⅰ, Est-Ⅱ and Est-Ⅲ in this experiment. Of the varieties analyzed, Japonica showed Est-Ⅰ and Indica was both Est-Ⅰ and Est-Ⅱ, while Japonica×Indica showed Est-Ⅲ, respectively. Esterase isozyme banding patterns of Tongil, Tongilchal, IR8, IR262, IR833, IR1317 and Peta were identical. It was concluded that the source of esterase isozymes genotype in grains of Tongil and Tongilchal was Indica variety Peta.