헤파린-플루로닉 나노젤의 세포내 흡입에 대한 키토산 코팅의 영향 = The Effect of Chitosan Coating on the Intracellular Uptake of Heparin-Pluronic Nanogels

다국어 초록 (Multilingual Abstract)

Chitosan was coated on the surface of a nanogel composed of heparin and Pluronic (HP nanogel) were investigated about internalizing property into cytosol. The chitosan-coated HP (CHP) nanogel was made via the self-assembly by increasing temperature in an aqueous solution. The average diameter of CHP and HP nanogels significantly increased and the surface charge was changed to more positive charge as increasing the feed amount of chitosan, which was measured by dynamic light scattering (DLS) and ζ-potential. Intracellular uptake study demonstrates that a large amount of CHP and HP nanogels was internalized intro the cytosol, which was performed by fluorescence activated cell sorter (FACS) and confocal laser scanning microscopy (CLSM). Contrary to our expectation, the kinetics of intracellular uptake was similar under varied nanogel concentrations and incubation times, regardless of chitosan coating.
In conclusion, obtained results demonstrate that chitosan coating on a HP nanogel does not affect significantly the intracellular uptake in spite of changed surface charges and hydrodynamic sizes.

참고문헌 (Reference)

1 W. E. Dager, "Treatment of heparin-induced thrombocytopenia" 36 : 489-503, 2002

2 J. K. Oh, "The development of microgels/nanogels for drug delivery applications" 33 : 448-477, 2008

3 D. H. Go, "Tetronic-oligolactide-heparin hydrogel as a multi-functional scaffold for tissue regeneration" 8 : 1152-1160, 2008

4 H. D. Park, "Surface modification of PMMA by chemical substitution of PEO derivatives and the effect on keratocyte adhesion" 3 : 122-128, 1999

5 N. Rapoport, "Stabilization and activation of Pluronic micelles for tumor-targeted drug delivery" 16 : 93-111, 1999

6 L. BrannonPeppas, "Nanoparticle and targeted systems for cancer therapy" 56 : 1649-1659, 2004

7 K. M. Park, "Nanoaggregate of thermosensitive chitosan-Pluronic for sustained release of hydrophobic drug" 63 : 1-6, 2008

8 박경민, "Injectable Chitosan-Pluronic Hydrogel Releasing Osteogenic Protein-1 for the Regeneration of Intervetebral Disc" 한국생체재료학회 12 (12): 24-28, 2008

9 N. Fatma, "Heparin’s role in stabilizing, potentiating, and transporting LEDGF into the nucleus" 41 : 2648-2657, 2000

10 J. S. Lee, "Heparin conjugated polymeric micelle for long-term delivery of basic fibroblast growth factor" 117 : 204-209, 2007

11 J. Panyam, "Dynamics of endocytosis and exocytosis of poly(D,L-lactide-co-glycolide) nanoparticles in vascular smooth muscle cells" 20 : 212-220, 2003

12 H. Hatakeyama, "Development of a novel systemic gene delivery system for cancer therapy with a tumor-specific cleavable PEG-lipid" 14 : 68-77, 2007

13 G. Borchard, "Chitosans for gene delivery" 52 : 145-150, 2001

14 M. Köping-Höggård, "Chitosan as a nonviral gene delivery system. Structure-property relationships and characteristics compared with polyethylenimine in vitro and after lung administration in vivo" 8 : 1108-1121, 2001