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    Insertional Mutagenesis and In Vivo Cloning-analysis of the Genes for the Defence against Oxygen

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    https://www.riss.kr/link?id=E685311

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    Eight Escherichia coli cells with aerobic growth defects were isolated by the insertion of ? placMu53, a hybrid bacteriophage of ? and Mu, which created transcriptienal fusion to lacZY. Mutants were tested for anaerobic expression of lacZ on XG medium for the possibilities of fusion to a promoter. Two of these mutants, CL10 and CL12, were irradiated with UV to get specialized transducing phages. Fifty-eight and twenty blue turbid plagues were picked for each insertion mutant. We identified the phages that have the chromosomal DNA of the related gene that is responsible for oxygen sensitivity. The in vivo cloned chromosomal sequence revealed that the mutated gene of CL10 is located at min 34.5 on Escherichia coli linkage map and 1,599,515 on physical map. We found that there are 7 cistrons in this operon.
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    Eight Escherichia coli cells with aerobic growth defects were isolated by the insertion of ? placMu53, a hybrid bacteriophage of ? and Mu, which created transcriptienal fusion to lacZY. Mutants were tested for anaerobic expression of lacZ on XG medium...

    Eight Escherichia coli cells with aerobic growth defects were isolated by the insertion of ? placMu53, a hybrid bacteriophage of ? and Mu, which created transcriptienal fusion to lacZY. Mutants were tested for anaerobic expression of lacZ on XG medium for the possibilities of fusion to a promoter. Two of these mutants, CL10 and CL12, were irradiated with UV to get specialized transducing phages. Fifty-eight and twenty blue turbid plagues were picked for each insertion mutant. We identified the phages that have the chromosomal DNA of the related gene that is responsible for oxygen sensitivity. The in vivo cloned chromosomal sequence revealed that the mutated gene of CL10 is located at min 34.5 on Escherichia coli linkage map and 1,599,515 on physical map. We found that there are 7 cistrons in this operon.

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