고정화 Mucor SPP L42 응유효소를 이용한 치즈커드 형성에 관한 연구 = Studies on coagulation of cheese curd by immobilized Mucor spp L42 milk clotting enzyme

국문 초록 (Abstract)

활성화시킨 Succinylaminopropyl glass beads에 glutaraldehyde을 사용하여 고정화시킨 Mucor spp L42 응유효소를 치즈 커드 형성과정에 이용하고져 고정화 효소의 안정성, 재이용성, 반응의 조건, 반응조의 형태등에 대하여 탈지유(pH 5.6, 8℃)를 사용하여 반응시킨후 30℃ 항온수조내에서 커드의 형성 상태를 조사하였던바 다음과 같은 결과를 얻었다.
1) 고정화 Mucor spp L42 응유효소를 0.2M phosphate buffer(pH 4.6)에 0.06% sodium azide을 첨가한 액에 침지한 상태로 5℃ 이하에서 보존할 경우 3개월 후에도 활력의 80%가 유지되었다.
2) 고정화 Mucor spp L42 응유효소를 탈지유(pH 5.6, 8℃)로 반응시켰을때 반응 초기에는 고정화 효소의 활력이 급격히 감소하였고, 이것은 bead에 결합되는 질소화합물과 효소의 활력간에 관계가 있는 것으로 나타났다.
3) 고정화 Mucor spp L42 응유효소에 bead와 탈지유의 접촉시간을 60 sec/ml로 하여 탈지유(pH 5.6, 8℃)로 연속적으로 반응시켰을때 6시간 이후에도 bead의 활력의 70%가 유지할 수 있었다.
4) Fluidized bed와 shaking fluidized bed을 사용하는 것이 충진반응조 보다 탈지유의 반응속도가 높았다.

다국어 초록 (Multilingual Abstract)

In order to study of practical purpose of immobilized Mucor spp L42 milk clotting enzyme on activated succimylamino-propyl glass beads with glutaraldehyde in continuous curd coagulation, acidified milk(pH5.6,8℃) was treated through reactor packed with immobilized beads, and warmed at 30℃ and allowed to coagulation for the determination of enzyme stability, deactivation of milk clotting ability by continuous reaction, the beads treatment conditions, and contact time of milk and beads in reactors.
The results obtained were summarized as follow;
1) After 3 month's storage, activity of immobilized Mucor spp L42 milk clotting enzyme in 0.2M phosphate buffer(pH 4.6) with 0.06% sodium azide was only 80% of initial activity.
2) Milk clotting activity of the beads was decreased by continuouse exposure on acidified skim milk. Nitrogen accumulation on the beads paralled loss of the activity in initial reaction stage.
3) After 6 hours continuous treatment of the beads at 60 sec/ml surface time, the milk-clotting activity of the beads was about 70% of initial activity.
4) Bead reactor and shaking bed reactor were more effective than column reactor on continuouse skim milk coagulation.