카드뮴에 의해 유발된 흰쥐 고환의 미세구조 및 단백질 변화 = Protein changes of the Rat Testis after Cadmium Administration

다국어 초록 (Multilingual Abstract)

Although cadmium is a well known heavy metal which attacks testis and results male infertility, the mechanism of action in the testis is'nt still fully known. In the previous experiment, authors observed morphological changes in the testis, and found cadmium-induced death of all the spermatogenic cells and hemorrhagic necrosis within the seminiferous tubules, and apoptosis and death of interstitial cells and fibroblasts in the interstitial tissue of the testis. In this experiment, cadmium chloride (4 mg/kg of body weight) administered to the adult male rats(Sprague-Dawlcy) intraperitoneally and sacrificed 1 week after treatment. And electrophoresis, immunoprecipitation and Western blotting, and N-terminal analysis were performed to find the proteins changed after cadmium treatment in the testis. And the results were as follows;
1. Two proteins of molecular weight 42kDa and 21kDa disappeared after cadmium treatment.
2. The protein of 2lkDa was rat collagen type I alpha 2.
According to the above results, it was considered that cadmium degraded the collagen fibers within the wall of small blood vessels in seminiferous tubules and interstitial tissues and disrupted vascular walls, which resulted hemorrhagic necrosis, death of all the spermatogenic cells, and the death of interstitial cells and fibroblastic cells.