Isolation of bio-active compounds from persimmon roots (Diospyros kaki) and development of methods to remove Methyl Chloride, a toxic industrial waste : 김혜민

다국어 초록 (Multilingual Abstract)

Part 1. Isolation of Bio-active Compounds from Persimmon roots (Diospyros kaki)
Persimmon (Diospyros kaki), which belongs to the Ebenaceae family, is widespread in Korea, Japan and China. Extract of persimmon roots showed antioxidant activity (IC_(50) ~50 ug/mL). Two novel naphthalene derivatives (4 and 6) and one novel naphthoquinone derivative (9) together with six known compounds (1-3, 5, 7-8) were isolated and identified from the plant Diospyros kaki. The structures of isolates were characterized by spectroscopic methods including UV, IR, HRTOFMS, ¹H NMR, ¹³C NMR, DEPT, 1D NOESY, gCOSY, gHSQC, gHMBC, and ROESY. The isolated compounds showed moderate antioxidant (IC_(50) 5.3-43.9 ug/mL) and cytotoxicity (IC_(50) 1.7-73.3 ug/mL) activity which were estimated by DPPH radical-scavenging assay and SRB assay, respectively. New compound 4 showed weak activity against Pityrosporum ovale.
Part 2. Development of methods to remove Methyl Chloride, a toxic industrial waste
A surfactant is a material absorbing to an interface in the watery solution and in which it reduces the surface tension. The quartenary amine cationic surface active agent occupies 80% of the cationic surface active agent market and the methyl chloride is used in order to make the quartenary amine into the trivalence amine. In this research, we developed an efficient method to remove unreacted methyl chloride from the quarternary amine cationic surface active agent by using the liquefied reaction method.
The speed of reaction with catalyst was faster than that of without catalyst. The reaction speed was also fast when organic solvent was used instead of water.
The faster reaction speed with high yield was found when the catalyst DDAC (di-hydrogenated tallow dimethyl ammonium chloride) and Aliquat 336 (Me(C_(8))₃NCl) were used.

목차 (Table of Contents)

• Part 1 Isolation of Bio-active Compounds from Persimmon roots (Diospyros kaki) = 1
• 1.1 Introduction = 2
• 1.2 Materials and Methods = 3
• 1.2.1 General experimental procedures = 3
• 1.2.2 Materials = 4
• 1.2.3 Extraction and Isolation = 5
• 1.2.4 Physicochemical Properties of the isolated compounds = 6
• 1.2.5 Procedure for free radical scavenging assay = 10
• 1.2.6 Procedure for cancer cell line cytotoxicity assay = 10
• 1.2.7 Hydrolysis of compound 6 = 10
• 1.3 Result and Discussion = 11
• 1.3.1 Structure determination of isolates = 12
• 1.3.2 Biological Activities of isolates = 22
• 1.3.2.1 Cytotoxicity and antioxidant activity = 22
• 1.3.2.2 Antimicrobial activity = 23
• 1.4 Conclusion = 23
• 1.5 Reference = 24
• Part 2 Development of methods to remove Methyl Chloride, a toxic industrial waste = 26
• 2.1 Introduction = 27
• 2.2 The experimental method and result = 27
• 2.2.1 The reactivity investigation of the model compound = 28
• 2.2.2 The reactivity investigation according to catalysts = 29
• 2.2.3 The reactivity investigation according to the use of organic solvent = 29
• 2.2.4 Methyl chloride elimination experiment = 31
• 2.3 Conclusion = 32
• 2.4 Reference = 33
• 3 Summary = 34
• 4 Appendices = 36
• 4.1 ¹H NMR spectrum of compound 4 = 36
• 4.2 ¹³C NMR spectrum of compound 4 = 36
• 4.3 gCOSY spectrum of compound 4 = 37
• 4.4 HMBC spectrum of compound 4 = 37
• 4.5 High resolution TOF mass data of compound 4 = 38
• 4.6 ¹H NMR spectrum of compound 6 = 38
• 4.7 ¹³C NMR spectrum of compound 6 = 39
• 4.8 gCOSY spectrum of compound 6 = 39
• 4.9 HMBC spectrum of compound 6 = 40
• 4.10 ROESYspectrum of compound 6 = 40
• 4.11 High resolution TOF mass data of compound 6 = 41
• 4.12 ¹H NMR spectrum of compound 9 = 41
• 4.13 ¹³C NMR spectrum of compound 9 = 42
• 4.14 gCOSY spectrum of compound 9 = 42
• 4.15 HMBC spectrum of compound 9 = 43
• 4.16 High resolution TOF mass data of compound 9 = 43