원추리 지상부의 최적추출조건 확립 및 추출물의 항산화 활성 = Determination of Optimum Extraction Solvent and Antioxidative Properties of Solvent Extract from Hemerocallis fulva Leaves

다국어 초록 (Multilingual Abstract)

This study was performed to investigate the possible utilization of Hemerocallis fulva (H. fulva) leaves as either functional food or natural antioxidants. To determine the optimum extraction solvent, the dried H. fulva was extracted with 7 solvents, and yield, total flavonoid content and 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity of each extract were compared. Among the extracts, 80% ethanol extract showed the highest yield, total flavonoid content and DPPH scavenging activity. The 80% ethanol extract was further fractionated subsequently by n-hexane, chloroform, ethyl acetate, n-butanol and water. Among five fractions from 80% ethanol extract, the ethyl acetate fraction showed the highest total flavonoid content (322.99 mg Que/g) among five fractions, and also exhibited an excellent DPPH scavenging activity (89.09∼93.79 at 100∼500 Ռg/ml). Metal-ion (10−3 M FeCl3) chelating effect of the ethyl acetate fraction at the concentration of 100 Ռg/ml showed 75.46%. The present study indicated that the ethyl acetate fraction exerted the highest total flavonoid content and the strongest antioxidative activity, suggesting that ethyl acetate fraction contains the bioactive compounds with antioxidative activity.

참고문헌 (Reference)

1 이광근, "바나바(Lagerstroemia speciosa L. Pars.) 잎 추출물의 분획 및 그들의 항산화 특성" 한국산업식품공학회 10 (10): 120-124, 2006

2 Konishi T, "Steroidal saponins from Hemerocallis fulva var. kwanso" 49 : 318-320, 2001

3 Minotti G, "Redox cycling of iron and lipid peroxidation" 27 : 219-, 1992

4 Hetog MGL, "Optimization of a quantitative HPLC determination of potentially anticarcinogenic flavonoids in vegetables and fruits" 40 : 1591-1598, 1992

5 Inoue T, "Novel 2,5-dihydrofuryl-γ-lactam derivatives from Hemerocallis fulva L. var. kwanzo Regel" 38 : 3187-3189, 1990

6 Zhang Y, "Lipid peroxidation inhibitory compounds from daylily (Hemerocallis fulva) leaves" 75 : 753-763, 2004

7 Cichewicz RH, "Isolation and characterization of stelladerol, a new antioxidant naphthalene glycoside, and other antioxidant glycosides from edible daylily (Hemerocallis) flowers" 50 : 87-91, 2002

8 Cichewicz RH, "Inhibition of human tumor cell proliferation by novel anthraquinones from daylilies" 74 : 1791-1799, 2004

9 Que F, "In vitro and vivo antioxidant activities of daylily flowers and the involvement of phenolic compounds" 16 : 196-203, 2007

10 Tobinaga S, "From my ethnopharmacochemical studies" 119 : 185-198, 1999

11 Solimani R, "Flavonoid-DNA interaction studied with flow linear dichroism technique" 43 : 876-882, 1995

12 Kim SK, "Evaluation of antioxidant activity" 3 : 35-39, 2008

13 Hsieh MT, "Effects of Hemerocallis fulva on motor activity and the concentration of central monoamines and its metabolites in rats" 52 : 71-76, 1996

14 Moreno MIN, "Comparison of the free radical-scavenging activity of propolis from several regions of Argentina" 71 : 109-114, 2000

15 State Administration of TCM, "Chinese meteria medica (Zhonghua bencao) 8" Shanghai Science & Technology Publishing House 101-106, 1997

16 Haraguchi H, "Antioxidative substances in leaves of Polygonum hydropiper" 40 : 1349-1351, 1992

17 Cha BC, "Antioxidative effect of domestic plants" 28 : 15-20, 1997

18 Lee JS, "Antioxidative characteristics of isolated crude phenolics from soybean fermentation foods (Doenjang)" 26 : 376-382, 1997

19 Blois MS, "Antioxidant determination by the use of a stable free radical" 26 : 1199-, 1958

20 Kanazawa K, "Antimutagenicity of flavones and flavonols to heterocyclic amines by specific and strong inhibition of cytochrome P450 1A family" 62 : 970-977, 1998

21 Inoue T, "2,5-dihydrofuryl- γ-lactam derivatives from Hemerocallis fulva L. var. Kwanso Regel. II" 42 : 154-155, 1994