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      피조개 양식장 내 용혈성 미생물의 다양성 분석 = Analysis of diversity of hemolytic microbiome from aquafarm of arkshell, Scapharca broughtonii

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      https://www.riss.kr/link?id=A99888229

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      다국어 초록 (Multilingual Abstract)

      The ark shell, Scapharca broughtonii is a marine bivalve mollusks belonging to the family Arcidae and important seafood for Korean and Japanese, and southern coast is brisk bays for the ark shell aquaculture. However, productivity of ark shell from these regions were rapidly reduced during the last decade due to mass mortality. The reason of this great damage has not yet been identified. To overcome this economic loss, diverse investigations were focused on environmental factors that affects in the physiology of S. broughtonii, but microbiological researches were performed insufficiently. Hemoglobin is one of the major blood component of ark shell and is damaged by some species of bacterial toxins. We concentrated on this red pigment because hemolysis could be the cause of ark shell mortality. In this study, we analyzed microbial diversity of underwater sediments in coastal regions and also existences in the body of S. broughtonii. We investigate about 4,200 isolates collected from June to September for microbial diversity of sediments and ark shell. We screened all of culturable microorganisms, and identified 25 genera 118 species, 24 genera 89 species, 30 genera 109 species and 39 genera 141 species, and selected 140 unique colonies for identification and challenge assay.
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      The ark shell, Scapharca broughtonii is a marine bivalve mollusks belonging to the family Arcidae and important seafood for Korean and Japanese, and southern coast is brisk bays for the ark shell aquaculture. However, productivity of ark shell from th...

      The ark shell, Scapharca broughtonii is a marine bivalve mollusks belonging to the family Arcidae and important seafood for Korean and Japanese, and southern coast is brisk bays for the ark shell aquaculture. However, productivity of ark shell from these regions were rapidly reduced during the last decade due to mass mortality. The reason of this great damage has not yet been identified. To overcome this economic loss, diverse investigations were focused on environmental factors that affects in the physiology of S. broughtonii, but microbiological researches were performed insufficiently. Hemoglobin is one of the major blood component of ark shell and is damaged by some species of bacterial toxins. We concentrated on this red pigment because hemolysis could be the cause of ark shell mortality. In this study, we analyzed microbial diversity of underwater sediments in coastal regions and also existences in the body of S. broughtonii. We investigate about 4,200 isolates collected from June to September for microbial diversity of sediments and ark shell. We screened all of culturable microorganisms, and identified 25 genera 118 species, 24 genera 89 species, 30 genera 109 species and 39 genera 141 species, and selected 140 unique colonies for identification and challenge assay.

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      참고문헌 (Reference)

      1 신윤경, "피조개의 생존과 대사에 미치는 수온, 염분 및 저산소의 영향" 한국패류학회 24 (24): 59-65, 2008

      2 김병학, "피조개, Scapharca broughtonii 부착치패의 중간양성시 보호망 형태와 유실방지망 종류에 따른 성장과 생존" 한국패류학회 24 (24): 131-136, 2008

      3 김동균, "패류(Scapharca broughtonii) 유래의 용혈활성 미생물 다양성 분석" 한국생명과학회 22 (22): 642-649, 2012

      4 김병학, "진해만 피조개 Scapharca broughtonii 양식장 식물플랑크톤의 종조성과 현존량의 월별변화" 한국양식학회 21 (21): 157-166, 2008

      5 WoRMS Editorial Board, "World register of marine species" VLIZ

      6 Woo, P.C., "Usefulness of the MicroSeq 500 16S ribosomal DNA-based significant bacterial isolates with ambigous biochemical profiles" 41 : 1996-2001, 2003

      7 Palleroni, N.J., "Prokaryotic diversity and the importance of culturing" 72 : 3-19, 1997

      8 Manns, J.M., "Production of a Hemolytic Factor by Candida albicans" 62 : 5154-5165, 1994

      9 Kelly, P.W., "Phylogeny of gammaproteobacteria" 192 : 2305-2314, 2010

      10 Thompson, F.L., "Phylogeny and molecular identification of vibrios on the basis of multilocus sequence analysis" 71 : 5107-5115, 2005

      1 신윤경, "피조개의 생존과 대사에 미치는 수온, 염분 및 저산소의 영향" 한국패류학회 24 (24): 59-65, 2008

      2 김병학, "피조개, Scapharca broughtonii 부착치패의 중간양성시 보호망 형태와 유실방지망 종류에 따른 성장과 생존" 한국패류학회 24 (24): 131-136, 2008

      3 김동균, "패류(Scapharca broughtonii) 유래의 용혈활성 미생물 다양성 분석" 한국생명과학회 22 (22): 642-649, 2012

      4 김병학, "진해만 피조개 Scapharca broughtonii 양식장 식물플랑크톤의 종조성과 현존량의 월별변화" 한국양식학회 21 (21): 157-166, 2008

      5 WoRMS Editorial Board, "World register of marine species" VLIZ

      6 Woo, P.C., "Usefulness of the MicroSeq 500 16S ribosomal DNA-based significant bacterial isolates with ambigous biochemical profiles" 41 : 1996-2001, 2003

      7 Palleroni, N.J., "Prokaryotic diversity and the importance of culturing" 72 : 3-19, 1997

      8 Manns, J.M., "Production of a Hemolytic Factor by Candida albicans" 62 : 5154-5165, 1994

      9 Kelly, P.W., "Phylogeny of gammaproteobacteria" 192 : 2305-2314, 2010

      10 Thompson, F.L., "Phylogeny and molecular identification of vibrios on the basis of multilocus sequence analysis" 71 : 5107-5115, 2005

      11 Montes, M.J., "Paenibacillus antarcticus sp. nov., a novel psychrotolerant organism from the Antarctic environment" 54 : 1521-1526, 2004

      12 Ash, C., "Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus" 64 : 253-260, 1993

      13 Donachie, S.P., "Microbial community in acidic hydrothermal waters of volcanically active White Island, New Zealand" 6 : 419-425, 2002

      14 Coenye, T., "Kerstersia gyiorum gen. nov., sp. nov., a novel Alcaligenes faecalis-like organism isolated from human clinical smaples, and reclassification of Alcaligenes denitrificans Rüger and Tan 1983 as Achromobacter denitrificans comb. nov" 53 : 1825-1831, 2003

      15 Stuart, P.D., "Idiomarina loihiensis sp. nov., a halophilic c-Proteobacterium from the Lō’ihi submarine volcano, Hawai’i" 53 : 1873-1879, 2003

      16 Rui, L., "Identification and characterization of pathogenic Vibrio splendidus from Yesso scallop (Patinopecten yessoensis) cultured in a low temperature environment" 114 : 144-150, 2013

      17 Kim, B.K., "Identification and characterization of metagenomic fragments from tidal flat sediment" 47 : 402-410, 1993

      18 Anju, P., "Hemolysin, protease, and EPS producing pathogenic Aeromonas hydrophila strain An4 shows antibacterial activity against marine bacterial fish pathogens" 2010 : 1-9, 2010

      19 Hoff, K.J., "Gene prediction in metagenomic fragments: a large scale machine learning approach" 9 : 217-230, 2008

      20 Chun, J.S., "EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences" 57 : 2259-2261, 2007

      21 Sorokin, V.A., "Evolutionary dynamics of clustered irregularly interspaced short palindromic repeat systems in the ocean metagenome" 76 : 2136-2144, 2010

      22 Park, M.S., "Effect of environmental foators on the growth, glycogen and hemoglobin content of cultured arkshell, Scapharaca broughtonii" 31 : 176-185, 1998

      23 McSpadden, Gardener, BB, "Ecology of Bacillus and Paenibacillus spp. in Agricultural Systems" 94 : 1252-1258, 2004

      24 Lal, S., "Ecology and biotechnological potential of Paenibacillus polymyxa" 49 : 2-10, 2009

      25 Topham, R., "Disulfide bond reduction: A powerful chemical probe for the study of structure-function relationships in the hemocyanin" 369 : 261-266, 1999

      26 Difco laboratories, "Culture media and ingredients, dehydrated" Difco laboratories. Division of Becton Dickinson and Company 302-303, 1998

      27 Cho, J.C., "Cultivation and Growth Characteristics of a Diverse Group of Oligotrophic Marine Gammaproteobacteria" 70 : 432-440, 2004

      28 Cuff, M.E., "Crystal structure of a functional unit from Octopus hemocyanin" 278 : 855-870, 1998

      29 Yoo, S.K., "Coastal culture" Gudeok publisher 203-218, 1986

      30 Suzuki, M.T., "Bacterial diversity among small-subunit rRNA gene cloned and cellular isolates from the same seawater sample" 63 : 983-989, 1997

      31 Paillard, C., "Bacterial disease in marine bivalves, a review of recent studies: trends and evolution" 17 : 477-498, 2004

      32 Schmidt, T.M., "Analysis of a marine picoplankton community by 16S rRNA gene cloning and sequencing" 173 : 4371-4375, 1991

      33 Weisburg, W.G., "16S ribosomal DNA amplification for phylogenetic study" 173 : 697-703, 1991

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