Association between SNP Marker of Uncoupling Protein 3 Gene and Meat Yield and Marbling Score Traits in Korean Cattle

다국어 초록 (Multilingual Abstract)

It is well established that uncoupling protein 3 (UCP3) is expressed largely in skeletal muscle, white adipose tissue and brown adipose tissue and has been suggested to play important roles in regulating energy expenditure, body weight, thermoregulation as well as fatty acid metabolism and obesity. Therefore, the UCP3 gene was selected as a candidate gene for carcass and meat quality traits in Korean cattle. The objective of this study was to identify single nucleotide polymorphisms (SNPs) in the UCP3 gene and to evaluate the association of UCP3 SNP markers with carcass and meat quality traits in Korean cattle. The five exons in the UCP3 gene were sequenced, and ten SNPs were identified. The PCR-SSCP method was then developed to genotype the individuals examined. The g.3076A>G genotype was significantly associated with marbling score (MS) of Korean cattle. Animals with the AA genotype had a higher MS than those with the AG and GG genotypes.
No significant associations of the SNP g.3076A>G were observed for any traits. In conclusion, although SNP g.3076A>G,which showed an association with MS, does not cause amino acid changes, this SNP may be used as a DNA marker to select animals that have higher intramuscular fat content.

참고문헌 (Reference)

1 Barendse, W., "Variationat CPE but not CEBPA appears to be associated withintramuscular fat deposition in the longissimus muscle ofcattle" 49 : 558-562, 2009

2 Van Eenennaam, A. L., "Validation of commercial DNA tests for quantitativebeef quality traits" 85 : 891-900, 2007

3 Boss, O., "Uncouplingprotein-3: A new member of the mitochondrial carrierfamily with tissue-specific expression" 408 : 9-42, 1997

4 Fleury, C., "Uncouplingprotein-2: a novel gene linked to obesity and hyperinsulinemia" 15 : 269-272, 1997

5 Vidal-Puig, A., "UCP3: an uncorpling protein homologueexpressed preferentially and abundantly in skeletal muscleand brown adipose tissue" 235 : 79-82, 1997

6 Zhao, J. G., "The study on the uncoupling proteingene as the candidate gene for fatness traits in chicken" 29 : 481-486, 2002

7 Wood, J. D., "The effect of cooking conditions on the eatingquality of pork" 40 : 127-135, 1995

8 Jian-Guo Zhao, "The Expression Characterization of Chicken Uncoupling Protein Gene" 아세아·태평양축산학회 18 (18): 1552-1556, 2005

9 Plastow, G. S., "Quality pork genes and meat production" 70 : 409-421, 2005

10 Sherman, E. L., "Polymorphismsand haplotypes in the bovine neuropeptide Y, growth hormonereceptor, ghrelin, insulin-like growth factor, anduncoupling proteins 2 and 3 genes and their associations withmeasures of growth, performance, feed efficiency, and carcassmerit in beef cattle" 86 : 1-16, 2008

11 Yamada, T., "Novel SNP in 5’flankinregion of EDG1 associated with marbling in Japanese Blackbeef cattle" 80 : 486-489, 2009

12 Haegeman, A., "Mapping and SNP analysis ofbovine candidate genes for meat and carcass quality" 34 : 349-353, 2003

13 J. D. Oh, "Identification of Novel SNPs with Effect on Economic Traits in Uncoupling Protein Gene of Korean Native Chicken" 아세아·태평양축산학회 19 (19): 1065-1070, 2006

14 Wang, Y. H., "Gene expression patterns duringintramuscular fat development in cattle" 87 : 119-130, 2009

15 Vidal-Puig, A., "Energy metabolismin uncorpling protein 3 gene knockout mice" 275 : 16258-16266, 2000

16 Katsumata, M., "Effect of heat exposure on uncoupling protein-3mRNA abundane in porcine skeletal muscle" 82 : 3493-3499, 2004

17 Gutierrez-Gil, B., "Detection ofquantitative trait loci for meat quality traits in cattle" 39 : 51-61, 2008

18 Matsuda, J., "Cloning of rat uncouplingprotein-3 and uncoupling protein-2 cDNAs: Their geneexpression in rate fedhigh-fat diet" 418 : 200-204, 1997

19 Stone, R. T., "Bovine UCP2 and UCP3 map to BTA15" 30 : 378-381, 1999

20 Otto, G., "Associationsof DNA markers with meat quality traits in pigs withemphasis on drip loss" 75 : 185-195, 2007

21 Gill, J. L., "Associations between single nucleotidepolymorphisms in multiple candidate genes and carcassand meat quality traits in a commercial Angus-crosspopulation" 86 : 985-993, 2010

22 Maj, A., "Association of the polymorphism in the 5'-noncodingregion of the bovine growth hormone receptor gene withmeat production traits in Polish Black-and-White cattle" 72 : 539-544, 2006

23 Miller, S. A., "A simplesalting out procedure or extracting DNA from humannucleated cell" 16 : 1215-, 1988