살무사(Agkistrodon blomhoffi brevicaudus)뱀독의 면역학적 성상 = Immunological Characteristics of Agkistrodon blomhoffi brevicaudus Venom

다국어 초록 (Multilingual Abstract)

This study was undertaken to find out main toxic components in venom of Agkistrodon b. brevicaudus and to prepare antiserum to venom. Through the experiment, venom of A. b. brevicaudus was fractionated into 7 fractions through DEAE-Sephadex A-50 and observation were made with toxic reaction of fractioned venom of A. b. brevicaudus, neutralizing effect of antiserum for highly toxic fraction and finally, comparison of neutralizing effect of antisera for fractionated venom and original crude venom.
The results ara summarized follows:
1. Fractionation of A. b. brevicaudus venom by DEAE sephadex A-50 column chromatograhpy resulted in seven peaks. The amount of total protein were the highest in 4th fraction.
2. To investigate the toxicity of each fraction, hemorrhagic activity of each fraction were compared. As a result, most strong hemorrhagic activity was shown in 6th fraction. Then 4th, 7th fraction were in that order.
3. Neutralizing effect of rat antisera for 2nd, 4th and 6th fraction were compared. Most effective neutralizing(antilethal) activity was shown in antiserum for 4th fraction and neutralizing effect of antiserum for 4th fraction was similar to that of crude venom.