HPV 진단검사방법 현황과 새로운 검사기술에 대한 탐색 = Current use and the latest trend in new technology development in human papillomavirus diagnostic methods

다국어 초록 (Multilingual Abstract)

High prevalence rate of human papillomavirus (HPV) has been one of the major health issues all over the world. Genital HPV is the most common sexually transmitted infection, and infects the skin and mucous membranes. Some HPV types can cause cervical cancer and other less common cancers, such as cancers of the vulva, vagina, anus, and penis. In this short review, current use and the latest trend in new technology development in HPV diagnostic methods were discussed. For cervical cancer diagnosis, Pap smear is employed as a preliminary test, and then one of nucleic acid detection methods such as Hybrid Capture^®2 and PCR is followed for the positive cases. PCR technology which amplifies target HPV sequence is usually combined with various advanced detection methods. Several trials with real time PCR products have not been satisfactory yet. However, a good success was made with Seeplex^® HPV 18-plex and Amplico^® plate which adopt multiplex PCR technology. HPV DNA microarray chips have drawn a great interest due to its speedy procedure, comparable specificity and sensitivity, and its potential in high throughput application. The use of newly approved HPV microarray chips including 4 products in Korea and Papillocheck^® chip in EU, Japan and Canada will be expanded soon for practical diagnostic use.