생쥐에 있어서 Ethanol 처리에 의한 단위발생의 유기 = Induction of Parthenogenetic Eggs by Ethanol Treatment in Mouse

다국어 초록 (Multilingual Abstract)

These experiments were carried out to obtain the basic informations and to establish the best conditions needed for the activation of mouse eggs by ethanol treatment. The influence of ethanol concentration and the age of eggs, time from hCG injection to recovery, on activation frequency of eggs was investigated, To activate the mouse eggs parthenogenetically, cumulus masses including eggs were released into a fresh solution of ethanol for 5min and then rinsed out 5-6 times with ethanol-free medium. After incubation of cumulus masses in the drops of medium for 5 hrs at 37℃ in the humidified atmosphere of 5% CO₂ in air, the adherent cumulus cells were removed with hyaluronidase (100unit/㎖). The types of parthenogenetic eggs were morphologically classified into haploid (H), immediate cleavage (IC) and diploid (D) under a inverted microscope. These parthenogenetic eggs were then incubated into the drops of mKRB medium for 96 hrs under the same conditions as above. The results obtained in these experiments were summarized as follows : 1. ICR female mice (1) When the eggs were treated with a variety of ethanol concentrations, 0 to 11%, the highest parthenogenetic activation rate (94.4%) was observed at 7% ethanol concentration. (2) Activation frequency was comparatively high when the eggs were recovered from the oviducts in more than 18 hrs after hCG injection. 2. (C₃H × C57BL) F₁ hybrid female mice (1) Of 341 F₁ eggs treated with 7% ethanol for 5 min at 18 hrs after hCG injection, 317 eggs (93.0%) were activated and a majority of parthenogenetic eggs were haploids (71.9%). (2) After 96 hrs of in vitro culture, the developmental rate of haploid, immediate cleavage and diploid eggs to morphologically normal morula or blastocyst stage was 16.7, 66.7 and 72.3%, respectively. (3) Of 18 haploid eggs recovered from the uteri on the 4th day of pregnancy after transfer to the oviducts of recipients, 12 eggs (60.0%) were normally developed to morula or blastocyst stage.