二次 口蓋組織 發生時 植物凝集素 表現에 關한 組織化學的 觀察 = A Histochemical Observation of Lectin Bindings on Developing Secondary Palate

다국어 초록 (Multilingual Abstract)

Carbohydrate rich macromolecules which have been demonstrated on the cell surface of a wide variety of animal cells have been implicated in behavior of the living cell including intercellular communication, the regulation of cell growth and differentiation, immune response, cellular recognition and adhesion during embryonic development. They also have been played an important role in secondary palatogenesis of developing mammalian fetuses but still little have been reported on their carbohydrate residues in detail yet. While, lectins have been known to combine specifically with sugar residues of such macromolecules on cell surfaces and hence bind cell together.
Therefore, the component of carbohydrate residues concerned with palatogenesis and their changes according to the process of palatal formation were investigated in DDY mouse using commercially available lectins, such as Concanavalin A (Con A), Ricinus communis agglutinin (RCA), wheat germ agglutinin (WGA), soybean agglutinin (SBA), Dolichos biflorus agglutinin (DBA) , peanut agglutinin (PNA), Pinellia ternata agglutinin (PTA) and Ulex europaeus agglutinin (UEA) by application of biotin-avidin peroxidase and benzidine substrate.
The results obtained are as follows:
1. Early period in palatal shelf formation, none but RCA showed light positive reaction to palatal epithelia and mesenchyme.
2 . Just prior to shelf elevation and reorientation, RCA and WGA revealed strong positive reaction to palatal tissue especially RCA on epithelia and WGA on mesenchyme, While Con A reacted lightly with both tissues.
3. Just prior to fusion after elevation, Con A showed strong positive reaction to epithelia especially on medial edge epithelia, whereas the positive reactivity with RCA and WGA was markedly reduced in both tissues.
4. The positive reactivity with Con A was rapidly decreased after palatal fusion.
5. SBA, DBA, PNA, PTA and UEA showed almost negative reaction to the developing palatal tissue through the palatogenesis.
With the results, it may be considered that macromolecules containing D-galactosyl residues and sialic acid could be related to the palatal shelf elevation, while materials known to appear in palatal shelf epithelia just prior to fusion would be considered as α- D-mannosyl and α-D-glucosyl residues containing macromolecules.