Influence of Repeated Senna Laxative Use on Skin Barrier Function in Mice = Influence of Repeated Senna Laxative Use on Skin Barrier Function in Mice

다국어 초록 (Multilingual Abstract)

Background: Senna, one of the major stimulant laxatives, is widely used for treating constipation. Chronic senna use has been reported to be associated with colonic disorders such as melanosis coli and/or epithelial hyperplasia. However, there is no obvious information on the influence of chronic senna use on organs except for the intestine. Objective: To clarify the influence of senna laxative use on skin barrier function by repeated senna administration. Methods: Eight-week-old male hairless mice received senna (10 mg/kg/day) for 21 days. After administration, we evaluated transepidermal water loss (TEWL), and investigated the bio-markers in plasma and skin using protein analysis methods. Results: Fecal water content on day seven was significantly increased; however, on day 21, it was significantly de-creased after repeated senna administration. In the senna-ad-ministered group, TEWL was significantly higher compared to the control on days seven and 21. Plasma acetylcholine concentration and NO₂ ^-/NO₃ ^- were increased on days sev-en and 21, respectively. In skin, tryptase-positive mast cells and inducible nitric oxide synthase (iNOS)-positive cells were increased on days seven and 21, respectively. The in-crease of TEWL on days seven and 21 was suppressed by the administration of atropine and N(G)-nitro-L-arginine methyl ester, respectively. Conclusion: It was suggested that diar-rhea or constipation induced by repeated senna admin-istration caused the impairment of skin barrier function. There is a possibility that this impaired skin barrier function occurred due to degranulation of mast cells via cholinergic signals or oxidative stress derived from iNOS. (Ann Dermatol 29(4) 414∼421, 2017)

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