새송이버섯 수확 후 배지로부터 surfactin 생성 Bacillus amyloliquefaciens YJ07의 분리 및 특성

다국어 초록 (Multilingual Abstract)

Spent mushroom substrates (SMS) is a by-product remaining after a crop of mushrooms. About four surfactin-producing strains were isolated from SMS (Pleurotus eryngii). Among of them, one isolate, which designated to YJ07, potentially showed the antifungal activity against Aspergillus flavus and Aspergillus ochraceous producing mycotoxin on PDA medium. The biochemical characteristics of the strain YJ07 was similar with Bacillus subtilis and Bacillus amyloliquefaciens by Bacillus ID kit and VITEK 2 system. Comparative 16S rDNA gene sequence analysis of the strain YJ07 also showed that the strain YJ07 was most closely related to Bacillus amyloliquefaciens with sequence similarity of 99.5%. On the basis of their biochemical characteristics and phylogenetic distinctiveness, the strain YJ07 was classified within the genus Bacillus as Bacillus amyloliquefaciens YJ07. The antifungal compound from B. amyloliquefaciens YJ07 was similar to lipopeptide surfactin from Bacillus subtilis by TLC and HPLC analysis.

목차 (Table of Contents)

• ABSTRACT
• 서론
• 재료 및 방법
• Surfactin 생성 균주의 분리
• Surfactin 생성 균주의 항균활성
• Surfactin 생성 균주의 동정
• 분리균이 생성하는 항균물질의 분리
• 결과 및 고찰
• 새송이버섯 수확 후 배지로부터 surfactin 생성 균주의 분리
• 분리균 YJ07의 항균활성
• 분리균 YJ07의 동정
• 분리균 YJ07이 생성하는 항균물질
• 적요
• 감사의 글
• 참고문헌

참고문헌 (Reference)

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