물레나물(Hypericum ascyron L.) 추출물의 생리활성 효소 억제 효과

국문 초록 (Abstract)

본 연구는 물레나물(Hypericum ascyron L.) 추출물을 사용하여 항산화, 미용식품 및 기능성식품 활성 등의 생리활성 효능을 평가하였다. 물레나물로부터 phenolic 화합물을 추출하기 위하여 물과 90% 에탄올로 추출하였을 때 각각 29.75, 31.82 mg/g의 페놀 함량을 나타내었다. 물레나물 추출물의 DPPH, ABTS 라디칼 소거능을 측정한 결과 물과 에탄올 추출물 100 μg/mL phenolic 농도에서 각각 86.90, 91.71%의 DPPH 전자공여능과 90.45, 98.05%의 ABTS 전자공여능을 나타내었다. Antioxidant protection factor(PF)는 물과 에탄올 추출물 50 μg/mL phenolics 농도에서 각각 2.11, 2.22 PF를 나타내었다. TBARS는 200 μg/mL phenolics 농도에서 물 추출물은 81.75%, 에탄올 추출물은 93.67%의 높은 항산화능을 나타내었다. α-Glucosidase 저해 효과를 측정한 결과 200 μg/mL phenolics 농도에서 물 추출물은 43.47%, 에탄올 추출물은 100.00%의 매우 높은 저해 효과를 나타내었다. Xanthine oxidase 저해효과를 측정한 결과 물과 에탄올 추출물 200 μg/mL phenolics 농도에서 각각 49.52, 85.30%의 저해 효과를 나타내었다. 미백 효과를 측정하는 tyrosinase 저해 효과를 측정한 결과 에탄올 추출물 200 μg/mL phenolics 농도에서만 89.59%의 저해 효과를 나타내었다. 주름개선 효과를 측정하는 collagenase, elastase 저해 효과를 측정한 결과 물 추출물에서는 200 μg/mL phenolics 농도에서 collagenase 저해 효과 측정에서만 84.76%의 저해 효과를 나타내었고, 에탄올 추출물에서는 200 μg/mL phenolics 농도에서 각각 98.46, 75.59%의 저해 효과를 나타내었다. 수렴 효과를 측정하는 astringent 효과를 측정한 결과 에탄올 추출물
200 μg/mL phenolics 농도에서만 88.23%의 astringent 효과를 나타내었다. 위의 결과에 따라 물레나물 추출물의 항산화, 당분해 억제, 항통풍, 미백, 주름개선 및 수렴 효과를 확인하였고, 기능성 소재로 이용 가능하다고 판단되었다.

다국어 초록 (Multilingual Abstract)

This study was designed to assess the anti-oxidation and biological activities of extracts from Hypericum ascyron L.. The contents of phenolic compounds in water and 90% ethanol extracts from H. ascyron L. were 29.75 and 31.82 mg/g, respectively. The 1,1-diphenyl-2-picrylhydrazyl free radical scavenging and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical decolorization activities of water and ethanol extracts were 86.90% and 91.71% as well as 90.45% and 98.05% at a phenolic content of 100 μg/mL, respectively. The antioxidant protection factor (PF) values of water and ethanol extracts at a phenolic content of 50 μg/mL were 2.11 PF and 2.22 PF, respectively. Thiobarbituric acid reactive substance values were 81.75% in water extract and 93.67% in ethanol extract at a phenolic content of 200 μg/mL. The inhibitory activities against α-glucosidase were 43.47% in water extract and 100.00% in ethanol extract at a phenolic content of 200 μg/mL. The inhibitory activities against xanthine oxidase in water and ethanol extracts were 49.52% and 85.30% at a phenolic content of 200 μg/mL, respectively. Tyrosinase inhibitory activities as a measure of whitening activity in ethanol extracts were 89.59% at a phenolic content of 200 μg/mL, respectively. The collagenase and elastase inhibitory activities as a measure of anti-wrinkle activity were 84.76% and not detected in water extract as well as 98.46% and 75.59% in ethanol extract at a phenolic content of 200 μg/mL, respectively. Astringent effect as a measure of pore tightening activity of the ethanol extracts were 88.23% at a phenolic content of 200 μg/mL, respectively. The results show that extracts from H. ascyron L. can be used as a functional resource with antioxidant, carbohydrate degradation inhibitory, anti-gout, whitening, anti-wrinkle, and pore tightening activities.

목차 (Table of Contents)

• ABSTRACT
• 서론
• 재료 및 방법
• 결과 및 고찰
• 요약
• REFERENCES

참고문헌 (Reference)

1 김영헌, "효모 Pichia burtonii Y257-7에 의한 α-Glucosidase 저해제의 생산 및 식후 혈당 상승 억제 효과" 한국미생물·생명공학회 42 (42): 219-224, 2014

2 이진태, "함초의 생리활성기능과 화장품소재로서의 응용" 대한본초학회 17 (17): 6-60, 2002

3 성낙주, "한약재 조성물의 항산화 활성 및 아질산염 소거능" 한국생명과학회 17 (17): 1135-1140, 2007

4 신정혜, "한약재 열수추출물의 항산화 활성" 한국식품영양과학회 35 (35): 7-14, 2006

5 이상진, "한방 이화주의 미백 및 피부 주름 개선 효과" 한국식품영양과학회 42 (42): 550-555, 2013

6 유아름, "생약 추출물의 RAW 264.7 세포를 이용한 면역증강 효과" 한국식품영양과학회 41 (41): 1521-1527, 2012

7 이진영, "도화(Prunus persica Flos) 분획물의 미백 및 주름개선 효과" 한국미생물·생명공학회 40 (40): 364-370, 2012

8 Wunsch E, "Zur quantitativen bestimmung der kollagenase" 333 : 149-151, 1963

9 Filha ZS, "Xanthine oxidase inhibitory activity of Lychnophora species from Brazil ("Arnica")" 107 : 79-82, 2006

10 Rittie L, "UV-light-induced signal cascades and skin aging" 1 : 705-720, 2002

11 Park SJ, "Two new species of Hypericum (Clusiaceae) from Korea" 15 : 458-463, 2005

12 Hanefeld M, "The role of acarbose in the treatment of non-insulin-dependent diabetes mellitus" 12 : 228-237, 1998

13 Stirpe F, "The regulation of rat liver xanthine oxidase" 244 : 3855-3863, 1969

14 Choe EA, "Studies on the xanthine oxidase inhibitor from bean sprouts" Yonsei University 1998

15 Wang ZQ, "Studies on the constituents of hong han lian (Hypericum ascyron L.)" 15 : 365-367, 1980

16 Lavie G, "Studies of the mechanisms of action of the antiretroviral agents hypericin and pseudohypericin" 86 : 5963-5967, 1989

17 Fellegrini N, "Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2,2'-azinobis(3-ethylenebenzothiazoline-6-sulfonic acid radical cation decolorization assay" 299 : 379-389, 1999

18 Borrello S, "Protective enzymes in human epidermal carcinomas and psoriasis" 276 : 338-340, 1984

19 Chung JH, "Photoaging in Asians" 19 : 109-121, 2003

20 Andarwulan N, "Phenolic content in differentiated tissue cultures of untransformed and Agrobacteriumtransformed roots of anise (Pimpinella anisum L.)" 47 : 1776-1780, 1999

21 Folin O, "On phosphotungstic-phosphomolybdic compounds as color reagents" 12 : 239-243, 1912

22 Frei B, "Natural antioxidants in human health and disease" Academic Press Publisher 40-55, 1994

23 Tibbot BK, "Molecular cloning and characterization of a gibberellin-inducible, putative ${\alpha}$-glucosidase gene from barley" 30 : 229-241, 1996

24 Buege JA, "Microsomal lipid peroxidation" 52 : 302-310, 1978

25 Hamilton AJ, "Melanins in fungal pathogens" 51 : 189-191, 2002

26 Hearing VJ Jr, "Mammalian monophenol monooxygenase (tyrosinase): purification, properties, and reactions catalyzed" 142 : 154-165, 1987

27 Saudek CD, "Lipid metabolism in diabetes mellitus" 66 : 843-852, 1979

28 Kim TJ, "Korean wild flower" Kyohaksa Publisher 330-, 1998

29 No JK, "Inhibition of tyrosinase by green tea components" 65 : PL241-PL246, 1999

30 Kraunsoe JA, "Inhibition of human leukocyte and porcine pancreatic elastase by homologues of bovine pancreatic trypsin inhibitor" 35 : 9090-9096, 1996

31 Brolis M, "Identification by high-performance liquid chromatography-diode array detection-mass spectrometry and quantification by high-performance liquid chromatography-UV absorbance detection of active constituents of Hypericum perforatum" 825 : 9-16, 1998

32 Im RJ, "Encyclopedia of Chosun medicinal plant III" Hankuk-munhwasa Publisher 147-, 1997

33 Cheon JH, "Effects of Backhousia citriodora extracts on antioxidant activity and bone formation" Silla University 2015

34 Chang SU, "Dictionary of Chinese crude drugs" New Medical College Shanghai Scientific Technological Publishers 283-, 1997

35 Ha BJ, "Cosmetic science" Soomoonsa Publisher 27-, 1999

36 Kil KS, "Antitumor activities of hypericin as a protein tyrosine kinase blocker" 19 : 490-496, 1996

37 Park YS, "Antioxidative activities and contents of polyphenolic compound of medicinal herb extracts" 12 : 23-31, 2002

38 Blois MS, "Antioxidant determinations by the use of a stable free radical" 181 : 1199-1200, 1958

39 Farag RS, "Antioxidant activity of some spice essential oils on linoleic acid oxidation in aqueous media" 66 : 792-799, 1989