저온성 Pseudomonas sp.에 의한 지방분해효소의 최적 생산조건에 관한 연구 = Studies on the Optimal Conditions for the Lipase Production of Psychrotrophic Pseudomonas sp.

다국어 초록 (Multilingual Abstract)

In order to isolate the bacteria which have lipolytic activities and to evaluate the effect of lipolytic activity on the milk and milk product quality, the raw milk samples were collected at several farms over Chon-Buk area. Each sample was spread on the tributyrin agar plates for the screening of lipase producing bacteria at 5℃ for 10days. Of the colonies grown on the tributyrin agar plate, an isolate B15 strain was selected for its strongest lipolytic activity and identified as a Genus of Pseudomonas sp. which was used for further studies. The cultural conditions for the lipase production were examined. As a result, the optimum cultural pH and temperature for the lipase production of Pseudomonas sp. were appeared at pH 8.0 and 25℃, respectively. Addition of 2.0%(w/v) starch as a carbon source and 0.5%(w/v) NaNO_(3) as a nitrogen source to the cultural medium was effective to the production of lipase.

목차 (Table of Contents)

• Ⅰ. 서론
• Ⅱ. 재료 및 방법
• Ⅲ. 결과 및 방법
• Ⅳ. 요약문
• 참고문헌