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    Generation of transgenic rice plants to improve heat tolerance : 애기장대 유래 비생물학적 스트레스 저항성 유전자들을 이용한 고온 저항성 벼 형질전환체 육성

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    https://www.riss.kr/link?id=T15076388

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    다국어 초록 (Multilingual Abstract) kakao i 다국어 번역

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    한국어
    Plants are exposed to a variety of environmental stresses as they grow, and these stresses affect plant growth. Global warming due to climate change and extreme weather change have a negative impact on agriculture and cause reduced crop yields. Thus, enhancing the plant’s abiotic stress resistance will improve plant production by maintaining plant growth and development even under severe environmental conditions. To enhance the high temperature stress resistance of rice, I investigated the abiotic polymorphism of HSP101 and CBF1 derived from the Arabidopsis thaliana by using the TDF1, OsLPS1, and UBQ14 promotors, which induce transgenic expression. Agrobacterium-mediated tissue culture was performed using the mature rice seeds of Dongjin to induce callus and transgenic rice plants were generated through regeneration process. Gene transfer was confirmed by PCR analysis of T0 and T1 plant genomic DNAs. These overexpression lines will be tested at vegetative stage and reproductive stages in the T2 generation. Studies on genes that are effective in promoting high temperature resistance of various candidate genes are expected to be useful as basic data for improving rice cultivars against global warming in the future.
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    Plants are exposed to a variety of environmental stresses as they grow, and these stresses affect plant growth. Global warming due to climate change and extreme weather change have a negative impact on agriculture and cause reduced crop yields. Thus, ...

    Plants are exposed to a variety of environmental stresses as they grow, and these stresses affect plant growth. Global warming due to climate change and extreme weather change have a negative impact on agriculture and cause reduced crop yields. Thus, enhancing the plant’s abiotic stress resistance will improve plant production by maintaining plant growth and development even under severe environmental conditions. To enhance the high temperature stress resistance of rice, I investigated the abiotic polymorphism of HSP101 and CBF1 derived from the Arabidopsis thaliana by using the TDF1, OsLPS1, and UBQ14 promotors, which induce transgenic expression. Agrobacterium-mediated tissue culture was performed using the mature rice seeds of Dongjin to induce callus and transgenic rice plants were generated through regeneration process. Gene transfer was confirmed by PCR analysis of T0 and T1 plant genomic DNAs. These overexpression lines will be tested at vegetative stage and reproductive stages in the T2 generation. Studies on genes that are effective in promoting high temperature resistance of various candidate genes are expected to be useful as basic data for improving rice cultivars against global warming in the future.

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    목차 (Table of Contents)

    • Introduction 1
    • Materials and Methods 4
    • Results and Discussion 8
    • References 23
    • Abstract (in Korean) 25
    • Introduction 1
    • Materials and Methods 4
    • Results and Discussion 8
    • References 23
    • Abstract (in Korean) 25
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