Non-native Metabolon-based Bioelectrocatalytic System Using Chimeric Invertase-Glucose Dehydrogenase = 인버타제-포도당탈수소효소 키메라를 활용한 비자연계 메타볼론 기반 전기촉매 시스템

다국어 초록 (Multilingual Abstract)

Enzymes are well recognized as very potent biocatalysts in the natural world, exerting a substantial
influence on several metabolic processes. Enzymes often exist in a state that might potentially exhibit increased
reaction efficiency inside the biological system via the formation of a multienzyme complex. In the present
work, an in vitro system was developed by constructing a non-native metabolon by the deliberate conjugation
of two enzymes originating from distinct sources. In this study, we have discovered the key parameters that
have the potential to significantly impact the efficiency of the reaction. Glucose dehydrogenase (GDHαγ) was
chosen as a representative enzyme due to its established credibility and extensively verified characteristics in
prior studies. The coupling enzyme used for this study was invertase (INV), which has the ability to create
glucose as an intermediate. In order to merge the two enzymes, a peptide spacer was used as a connecting
element between GDHαγ and INV. The catalytic activity was significantly impacted by the expression order of
GDHαγ and INV, and the catalytic activity exhibited notable variations based on the spacer sequence. The
criticality of regulating the distance and orientation between the coupling enzymes in a cascade reaction has
been identified.